M.Sc. (Semester II) (CBCS) Examination Nov/Dec-2018
Biotechnology
MOLECULAR CELL PROCESSING
Time: 2½ Hours Max. Marks: 70
Instructions: All questions of Section I is compulsory.
Answer any four questions from Section II.
All questions carry equal marks.
Draw neat and labeled diagrams wherever necessary.
SECTION I
Q.1 Rewrite the sentences by choosing correct answer from given
alternatives:
07
DNA polymerase I plays important role during
Replication
ii) Transcription
iii) DNA repairs
Only i Both i and iii
Both ii and iii Only ii
In the experiment of Meselson and Stahl the sole source of N15 isotope in
culture media was
NH4CI Glycine
Guanine Cytosine
The sense strand is also called as
primer strand template strand
coding strand antisense strand
Transcription factor H (TFIIH) has activity.
Polymerase DNA helicase
Exonuclease Endonuclease
Calcitonin and CGRP are produced by alternative splicing of pre-mRNA in
respectively.
Thyroid and neurons of CNS Thyroid and liver
Liver and neurons of CNS Neurons of CNS and thyroid
Aminoacyl tRNA synthetase is also called as
tRNA mutase tRNA ligase
tRNA transferase tRNA isomerase
Synthesis of is the role of RNA polymerase III.
mRNA primer
micro RNA tRNA
Define the terms:- 07
1. RecA
2. Sigma factor
3. snRNA
4. Satellite DNA
5. Polyadenylation
6. DNA gyrase
7. SOS repair
Page 2 of 2
SLR-VE-77
SECTION II
Answer any four of the following:-
Q.2 Describe the process of translation in eukaryotes. 14
Q.3 Explain in details the process of replication in prokaryotes. 14
Q.4 Describe the process of transcription in prokaryotes. 14
Q.5 Answer any two of the following:- 14
Describe mechanism of recombination
Describe prokaryote and eukaryote genome organization.
What are causes of DNA damage? Explain mismatch repair.
Q.6 Write notes on (any 14
trp operon
DNA as genetic material
Characteristics of genetic code.