Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharm. SEMESTER VI • EXAMINATION WINTER • 2016
Subject Code: 260002 Date: 21-10-2016
Subject Name: Pharmaceutical Microbiology and Biotechnology II
Time: 02:30 pm 05:30 pm Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

What is gene cloning? Describe gene cloning by restriction endonulease.
06

Discuss scope of biotechnology.
05

Write a detailed note on Transformation.
05
Q.2

What is mutation? Enlist types of mutation. Write a note on mutagens.
06

Write a short note on Replica plate method to identify the mutants.
05

Write a note on humulin.
05
Q.3

Differentiate: Active and Passive Immunity Toxin and Toxoid
06

Classify vaccines. Write a note on production of live bacterial vaccine.
05

Write a note on Schick test.
05
Q.4

Write a note on Immunoglobulins.
06

Comment Second line defense is nonspecific defense.
TAB is mixed polyvalent vaccine.
05

Write a note on serological reactions.
05
Q.5

Draw well labeled diagram of a fermenter. Describe its parts in brief.
06

Discuss upstream processing for fermentation of Penicillin.
05

Discuss downstream processing of fermentation on Vitamin B12.
05
Q. 6

Comment: Liquid media is preferred in large scale fermenters.
Seed culture is used for large scale fermentation.
06

What is importance of primary and secondary screening in fermentation?
Describe any one of primary screening method.
05

Give flow sheet for production of streptomycin by fermentation.
05
Q.7

What is immobilization of enzymes? Give its advantages and applications.
06

Enlist techniques of Immobilization of enzymes. Discuss any two in detail.
05

Discuss factors affecting enzyme kinetics.
05