Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM SEMESTER- VI EXAMINATION SUMMER-2017
Subject Code: 260002 Date:01/05/2017
Subject Name: Pharmaceutical Microbiology and Biotechnology II
Time: 10.30 AM to 1.30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Define Biotechnology. Discuss scope and application in the field of Pharmacy.
06

Describe bacterial variation with suitable example.
05

Enumerate different types of mutations. Explain in details any one.
05
Q.2

Define Genetic Recombination. Write a short note on Protoplast fusion.
06

How humulin is prepared biotechnological.
05

How monoclonal antibodies are produced?
05
Q.3

What is antigen? Write a detail note on Immunoglobulins.
06

Classify different types of immunity with example.
05

Explain the method of production and standardization of BCG vaccine.
05
Q.4

Describe antigen antibody reactions and its application.
06

Describe the various steps involved in gene cloning with suitable diagram
05

Write difference between Vaccine Sera, Active Passive immunity.
05
Q.5

Describe in detail the design of ideal fermenter with a labeled diagram.
06

Describe methods for preparation and maintenances of master culture in industries.
05

Give the flow chart for the production of penicillin.
05
Q. 6

Write a note on recombinant DNA technology.
06

Explain Polymerase Chain Reaction in detail.
05

Write short notes on fermentation media and pretreatments.
05
Q.7

What is enzyme? Explain in detail enzyme kinetics
06

Describe surface immobilization by covalent coupling.
05

Give applications of Immobilized enzymes in pharmacy
05