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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034 M.Sc.DEGREE EXAMINATION MEDICAL LAB TECHNOLOGY FOURTHSEMESTER APRIL 2017
ML 4815- SEPARATION TECH. PHARMACEUTICAL CHEMISTRY
Date: 20-04-2017 Dept. No. Max. 100 Marks
Time:09:00-12:00 PART A Answer ALL the questions (10 x 2 20 Marks)
1. What is first -pass drug effect?
2. Comment on Agonists and antagonists
3. Name the different types of centrifuge rotors.
4. Differentiate southern from northern blotting.
5. What is Gradient elution?
6. Name the detectors used in the TLC.
7. Define the term pharmacodynamics.
8. What is partition coefficient?
9. Expand the abbreviations: SCOT WCOT.
10. What is molecular probe?
PART B Answer any FOUR of the following x 10 40 Marks)
11. Briefly explain the mechanism of drug excretion.
12. Write notes on column chromatography with suitable diagram.
13. Classify and give detailed description of density gradient centrifugation. 14. Enumerate the different types of drug receptors.
15. Give an account on the DNA blotting technique.
16. Write notes on ion exchange chromatography.
PART C Answer any TWO of the following x 20 40 Marks)
17. Discuss in detail the mechanism of drug action.
18. Elaborate the principal routes of drug administration.
19. Draw and explain the principle and uses of HPLC.
20. Describe in detail the principle, procedure and instrumentation of SDS-PAGE.