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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc.DEGREE EXAMINATION MEDICAL LAB TECHNOLOGY
FOURTHSEMESTER APRIL 2018
16PML4MC02/ML4815 SEPARATION TECHNIQ. AND PHARMACEUTICAL CHEM.
Date: 20-04-2018 Dept. No. Max. 100 Marks
Time: 01:00-04:00
PART-A
Answer ALL the questions (10 X 2=20 Marks)
1. Name the most commonly used carrier gases in GLC.
2. Mention the different forces of centrifugal.
3. Comment on ligands.
4. List the different methods of column elution techniques.
5. What is drug half-life?
6. Write the importance of Restriction endonucleases.
7. Differentiate pharmacokinetics from pharmacodynamics.
8. Draw a neat labelled diagram of TLC.
9. Name the membrane used in blotting techniques.
10. What is gradient elution?
PART-B
Answer any FOUR of the following X 10 =40 Marks)
11. Elaborate the mechanism of analytical ultracentrifugation.
12. Explain the different types of centrifuge rotors with suitable diagram.
13. Write short notes on column chromatography.
14. What is blotting? Explain northern blotting techniques with suitable diagram.
15. Describe the different routes of drug excretion.
16. Give an account on the pharmacodynamics.
PART-C
Answer any TWO of the following X 20=40 Marks)
17. Write down the principle, procedure and instrumentation of HPLC.
18. What is electrophoretic mobility? Explain SDS and PAGE.
19. Elaborate the principle routes of drug administration with example.
20. Discuss in detail the analytical ultracentrifugation technique.