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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc. DEGREE EXAMINATION MEDICAL LAB TECHNOLOGY
FOURTH SEMESTER NOVEMBER 2017
ML 4815 SEPARATION TECH. PHARMACEUTICAL CHEMISTRY
Date: 03-11-2017 Dept. No. Max. 100 Marks
Time: 09:00-12:00
PART A
Answer ALL questions 10x2=20 marks
1. What is sedimentation co-efficient?
2. Mention the detectors used in TLC.
3. Comment on "Molecular scissors"
4. Differentiate pharmacokinetics from pharmacodynamics.
5. What is molecular probe?
6. Name any four commonly used ligands in affinity chromatography.
7. Define: Retention time Retention volume.
8. Comment on autoradiography.
9. What is bioavailability of drug?
10. Write the composition of sephadex.
PART B
Answer any FOUR of the following 4x10=40 marks
11. Classify and explain the density gradient centrifugation.
12. Write a paragraph on ion exchange chromatography.
13. Enumerate the importance of Northern blotting techniques.
14. Draw and explain the different centrifuge rotors.
15. Discuss the mechanism of drug absorption.
16. Describe the steps involved in the isolation and separation of human DNA.
PART C
Answer any TWO of the following 2x20=40 marks
17. Elaborate the principle routes of drug administration.
18. Describe in detail the principle, procedure and instrumentation of GLC.
19. Explain the mechanism of differential centrifugation with an illustration.
20. Elaborate the principle, procedure and clinical applications of PCR.