M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Bioinformatics
ADVANCED MOLECULAR BIOLOGY
Day Date: Saturday, 25-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: Part Question 1 is compulsory.
Attempt any four questions from part II.
Figures to the right indicate full marks.
Answer to the Part-I and Part II are to be written in same answer Booklet
only.
Part I
Q.1 Rewrite the sentences after choosing the correct answer from the
given alternatives.
07
Western Blotting is used for
DNA Protein
RNA Both a b
PCR is used in
RAPD AFLP
RFLP Both a b
cDNA is prepared from
rRNA mRNA
tRNA snRNA
Dialysis is used to remove
Salt Protein
Both a b None
Edman degradation is used for
DNA sequencing Protein sequencing
RNA sequencing All
DNA fingerprint is used for detect
Paternity Crime samples
Genetics test All
chromatography is used to separate proteins based on size
and shape.
HPLC Ion exchange
Affinity Gel filtration
Definition: 07
Autoradiography
Taq DNA Polymerase
Biochip
ddNTPs
Cy3 Cy5
Nitrocellulose membrane
RAPD
Page 2 of 2
SLR-E-27
Part II
Answer any four of the following:
Q.2 Define PCR. Explain different types of PCR techniques. 14
Q.3 Explain in detail the protein isolation and concentration. 14
Q.4 What is electrophoresis? Describe the separation of proteins using SDS PAGE. 14
Q.5 Answer any two of the following: 14
Write a note on Plaque hybridization technique.
Add a note on DNA finger printing.
Write a note on principle of column chromatography.
Q.6 Write short notes on (any 14
Iso Electric Focusing.
RT-PCR.
Molecular markers.