M.Sc. Bioinformatics (Semester-IV) (CBCS) Examination, 2017
ADVANCED MOLECULAR BIOLOGY
Day Date: Monday, 24-04-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
N.B. Part-1, Question 1 is compulsory.
Attempt any four questions from part-II.
3 Figures to the right indicate full marks.
Attempts any two questions from Q.5 to Q.7.
Answers to the Part-I and Part-II are to be written in
same answer booklet only.
PART-I
Q.1 Rewrite the sentence after choosing the correct answer
from the give alternatives:
07
Western Blotting is used for
DNA Protein
RNA Both a b
tool is used to analyze peptide mass fingerprint.
Mass spectroscopy MASCOT
ExPassy All
Molecular based diagnosis is done using instrument.
FTIR PCR
UV-VIS All
Localization of gene in cell or tissue is used.
In vitro In Vivo
In Situ All
technique is used to separate proteins in its native
from.
SDS PAGE Native PAGE
IEF 2D PAGE
technique is used to identify recombinant phages.
In situ hybridizatio Colony hybridization
Plaque hybridization None
Molecular marker is used to study the polymorphism
in genetic material using PCR.
Western Blot RAPD
RFLP Both b C
Page 1 of 2
Answer the following 07
Molecular Markers
Nitrocellulose membrane
Dansyl chloride
Dialysis
Plaque Hybridization
Microarray
Authoradiography
PART-II
Q.2 Explain the Primer designing parameters using Oligo 4 software. 14
Q.3 Explain in detail the instrumentation an applications of HPLC and
GLC.
14
Q.4 Explain protein sequencing by Edman degradation method. 14
Q.5 Answer any two of the following. 14
Describe variable number tandem repeat (VNTR).
Write a note on affinity chromatography with neat labeled
diagram.
Describe 2D gel electrophoresis.
Q.6 Short notes (Any 14
SNP
cDNA Library
Site Directed Mutagenesis.