M.Sc. (Semester III) Examination Nov/Dec-2018
Bioinformatics
ADVANCED MOLECULAR BIOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to right indicate full marks.
Q.1 Complete the sentences by selecting correct answer from the given
alternatives:
14
PCR technique was developed by
Kary Mullis Kolhar
Milstein Altmaz
PCR is a
DNA degradation technique DNA amplification technique
DNA sequencing technique DNA restriction technique
Aminobenzyloxymethyl filter paper is commonly used in
Western blotting Southern blotting
Northern blotting Dot blotting
Labeled antibodies are used to detect presence of particular
molecule in blotting.
RNA DNA
Protein mRNA
Agarose is composed of
Protein Vitamins
Carbohydrates RNA
In gel electrophoresis fragments separated on the basis of
Charge Temperature sensitivity
Absorption Adsorption
Factor does not affect the formation of hybrid DNA.
Ionic strength Temperature
Pressure Homologous DNA
Probe is a
Chemically synthesized DNA
Purified DNA
Fragmented DNA duplex
Either purified or synthesized single stranded DNA
Genomic library is normally made by
Alpha phage vector Lambda phage vector
Beta phage vector Gyamma phage vector
10) During electrophoresis denaturation of double stranded DNA is brought
about by
Treatment with alkali Application of current
Treatment with EtBr Application of heat
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11) Dialysis is used to remove
Salt Protein
Dust Buffer
12) Edman degradation is used for
DNA sequencing Protein sequencing
RNA sequencing Sugar sequencing
13) DNA fingerprint is not used to detect
Paternity Crime samples
Genetic test Protein
14) chromatography is used to separate proteins based on size and
shape.
HPLC Ion exchange
Affinity Gel filtration
Q.2 Answer the following (any four) 08
Define primer.
What is meant by blotting?
Define molecular marker and enlist examples.
Define electrophoresis?
Define probe and its types.
Write a notes on (any two) 06
Write a note on reverse transcriptase PCR.
Explain application of Blotting.
Explain single nucleotide polymorphism.
Q.3 Answer the following (any two) 08
Write a note on site directed mutagenesis.
Explain in detail cDNA library.
Write a note on basic principle of electrophoresis.
Answer the following (any one) 06
Write a note on shotgun method.
Write a short note on principle of ion exchange chromatography.
Q.4 Answer the following (any two) 10
Explain in detail Real time PCR.
What is protein purification? Explain in detail ammonium salt
precipitation.
Write an account on affinity chromatography.
Answer the following (any one) 04
Explain in detail Western blotting.
Briefly explain working of gel filtration chromatography.
Q.5 Answer the following (any two) 14
Define hybridization. Explain in brief colony hybridization with neat labeled
diagram.
Write detail account on HPLC.
Explain methods of protein sequencing.