B.Sc. I (Entrepreneurship) (CBCS) (Semester Examination, 2018
INDUSTRIAL MICROBIOLOGY I
Basic Techniques in Microbiology
Day and Date Friday, 6-4-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
N.B. All questions are compulsory and carry equal marks.
Draw a neat labeled diagram wherever necessary.
Figure to the right indicates full marks.
1. Rewrite the following sentences by choosing correct alternatives from given
below 14
Father of Microbiology is
Lister A. V. Leeuwenhoek
Louis Pasteur Robert Koch
ii) The refractive index of air is
0.50 0.75 1.0 1.25
iii) is used as a secondary stain in acid fast staining of
mycobacterium.
Crystal violet Giemsa
Methylene blue Carbol fuchsin
iv) is used for cell enumeration.
Coulter Counter Calibrator
Chromatographic chamber Refractometer
Endospore can be stained with
Safranine Crystal violet
Methylene blue ZNCF
vi) Solidifying agent commonly used in media preparation is
Agar-agar Silica gel Gel well Soft gel
vii) Total viable plate count can be obtained using technique.
Spread plate Pour plate
Both and Hemocytometer
viii) All the following are components of compound microscope expect
Stage clips Fine adjustment
Electron gun Binocular eye piece
ix) Electron microscope gives magnification up to
100 X 2000 X 50,000 X 2,00,000 X
Acid fast staining is an example of staining.
Simple Monochrome Differential None of these
xi) is selective component for coliforms used in MacConkeys
agar.
NaCl Peptone
Sodium taurocholate Agar
xii) The characteristic shape of the bacteria is maintained because of
Capsule Cell wall
Cell membrane Slime layer
xiii) The resolution power of the compound microscope is
0.2 micron 0.2 millimeter
0.2 Angstrom 0.2 centimeter
xiv) Primary stain is used in Gram staining is
Iodine Crystal Violet Safranin Acetocarmine
2. Answer any seven of the following 14
Define differential staining.
ii) What is differential media
iii) Enlist example of acidic stain.
iv) Applications of SEM.
Define non-living media.
vi) Neubaurs chamber.
vii) TVC.
viii) Enlist component of Semi-synthetic media.
ix) Role of Bile salt.
3. Answer any two of the following 10
Describe in brief acid fast staining.
ii) Discuss in detail construction and working of electron microscope.
iii) Describe in detail indirect methods of cell enumeration.
Give a detailed account on component of culture media. 4
4. Answer any two of the following 14
Describe in detail cultivation and isolation techniques.
ii) Define stain and explain in detail classification of stain.
iii) Explain in detail non-living media.
5. Answer any two of the following 14
Write in detail Gram staining.
ii) Discuss in detail living media.
iii) Describe in detail construction, working and principal of bright field
microscopy.