Exam Details
| Subject | basic techniques in microbiology | |
| Paper | ||
| Exam / Course | b.sc. – i (entrepreneurship) | |
| Department | ||
| Organization | solapur university | |
| Position | ||
| Exam Date | October, 2018 | |
| City, State | maharashtra, solapur |
Question Paper
B.Sc. I (Semester II) (Entrepreneurship) (CBCS) Examination, 2018
industrial microbiology II
Basics Techniques in Industrial Microbiology
Day and Date Tuesday, 27-11-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Choose the correct alternative and rewrite the sentences again. 14
can be used for strain improvement.
Mutagenesis Site directed mutagenesis
Gene manipulations All of these
ii) Ethyl Methane Sulfonate and Ethidium Bromide are
mutagenic agents.
Physical Chemical
Biological rDNA
iii) UV is mutagenic agent.
Physical Chemical
Biological rDNA
iv) rDNA technology is also known
Mutation Gene manipulation
Fermentation Sterilization
Gradual process of enhancing lab fermentations to industrial level is known
as
Up stream Down stream
Scale up Scale down
Set P
SLR-SK 17 *SLRSK17*
Set P
vi) Scale up of fermentation considers
Fermentation economics Upstream processing
Downstream processing All of these
vii) Microbial preparation for addition in fermentation medium is known as
Media optimization Inoculums preparation
Mutation Scale down
viii) End point assay is practiced for determination of
Vitamin Antibiotic
Growth factor Citric acid
ix) Auxotrophic mutants are used for assay.
Vitamin Antibiotic
Citric acid Ethanol
Determination of optical density of broth is practiced in
assay.
Enzymatic Diffusion
Turbidometric metabolic response
xi) Speed of agitation is measured using unit.
RPM RSM RTM RAM
xii) The recovery and purification of fermentation products refers
Upstream processing Downstream processing
Incubation Inoculation
xiii) On the basis of boiling point volatile compounds are purified using
Fractional distillation Centrifugation
Filtration Precipitation
xiv) In computer based control, manually or automatic of the
fermenter are controllled.
Valves of auxiliary equipments Media composition
Product formation Downstream processing
*SLRSK17* SLR-SK 17
Set P
2. Define and explain in short any seven of the following 14
Mutation.
ii) rDNA technology.
iii) What is filtration
iv) Assay.
Metabolites.
vi) Distillation.
vii) Centrifugation.
viii) Turbidity.
ix) Sparger.
3. Answer any two of the following. 10
Write in detail on inoculum preparation.
ii) Explain in brief metabolic response assay.
iii) Discuss in brief downstream processing using filtration and
centrifugation.
Give brief account on strain improvement using mutagenesis. 4
4. Answer any two of the following 14
Write an essay on computer applications in fermentation technology.
ii) Write an essay on downstream processing using solvent extraction and
distillation.
iii) Write an essay on downstream processing using precipitation and
crystallization.
5. Answer any two of the following 14
Write an essay on scale up of fermentation.
ii) Write an essay on enzymatic assay.
iii) Write an essay on strain improvement using gene manipulation.
industrial microbiology II
Basics Techniques in Industrial Microbiology
Day and Date Tuesday, 27-11-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Choose the correct alternative and rewrite the sentences again. 14
can be used for strain improvement.
Mutagenesis Site directed mutagenesis
Gene manipulations All of these
ii) Ethyl Methane Sulfonate and Ethidium Bromide are
mutagenic agents.
Physical Chemical
Biological rDNA
iii) UV is mutagenic agent.
Physical Chemical
Biological rDNA
iv) rDNA technology is also known
Mutation Gene manipulation
Fermentation Sterilization
Gradual process of enhancing lab fermentations to industrial level is known
as
Up stream Down stream
Scale up Scale down
Set P
SLR-SK 17 *SLRSK17*
Set P
vi) Scale up of fermentation considers
Fermentation economics Upstream processing
Downstream processing All of these
vii) Microbial preparation for addition in fermentation medium is known as
Media optimization Inoculums preparation
Mutation Scale down
viii) End point assay is practiced for determination of
Vitamin Antibiotic
Growth factor Citric acid
ix) Auxotrophic mutants are used for assay.
Vitamin Antibiotic
Citric acid Ethanol
Determination of optical density of broth is practiced in
assay.
Enzymatic Diffusion
Turbidometric metabolic response
xi) Speed of agitation is measured using unit.
RPM RSM RTM RAM
xii) The recovery and purification of fermentation products refers
Upstream processing Downstream processing
Incubation Inoculation
xiii) On the basis of boiling point volatile compounds are purified using
Fractional distillation Centrifugation
Filtration Precipitation
xiv) In computer based control, manually or automatic of the
fermenter are controllled.
Valves of auxiliary equipments Media composition
Product formation Downstream processing
*SLRSK17* SLR-SK 17
Set P
2. Define and explain in short any seven of the following 14
Mutation.
ii) rDNA technology.
iii) What is filtration
iv) Assay.
Metabolites.
vi) Distillation.
vii) Centrifugation.
viii) Turbidity.
ix) Sparger.
3. Answer any two of the following. 10
Write in detail on inoculum preparation.
ii) Explain in brief metabolic response assay.
iii) Discuss in brief downstream processing using filtration and
centrifugation.
Give brief account on strain improvement using mutagenesis. 4
4. Answer any two of the following 14
Write an essay on computer applications in fermentation technology.
ii) Write an essay on downstream processing using solvent extraction and
distillation.
iii) Write an essay on downstream processing using precipitation and
crystallization.
5. Answer any two of the following 14
Write an essay on scale up of fermentation.
ii) Write an essay on enzymatic assay.
iii) Write an essay on strain improvement using gene manipulation.
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