B.Sc. I (Entrepreneurship) (Semester (CBCS) Examination, 2018
industrial microbiology i
Basic Techniques in Microbiology
Day and Date Monday, 12-11-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
N.B. All questions are compulsory and carry equal marks.
Draw a neat labelled diagram wherever necessary.
Figure to the right indicates full marks.
1. Rewrite the following sentences by choosing correct alternatives from given
below 14
is used to visualize the surface view of object.
TEM SEM
Dark field Compound
The process of obtaining growth of microorganism in artificial environment
is called as
Cultivation Isolation
Screening Inhibition
The population of more than one type of microorganism is known as
culture.
Pure Synchronous
Axenic Mixed
staining is example of differential staining used to stain
mycobacterial strain.
Acid-Fast Grams
Cell wall Capsule
The total cell count is carried out by method for enumeration of
growth.
SPC DMC
MPN Membrane filter
The ability of lens system to distinguish two adjacent points as distinct and
separate is called as
Magnification Numerical aperture
Resolving power Condenser
used as mordant in Gram staining.
Alcohol Grams iodine
Bouins fixative Tannic acid
stain contains negative charged chromophore group.
Acidic Basic
Neutral Ramanovsky
is the direct method used for enumeration of bacteria in the
supension.
SPC Electronic counters
Turbidity Filtration
10) Mac'Conckeys agar is a example of media.
Selective Differential
Both and Enriched
11) Continuous cell culture used for cultivation of virus is example of
of media.
living non living
synthetic complex
12) stain contains both positive and negative charged chromophore
groups.
Cango red Saffranine
Giemsa Nigrosine
13) The media which is composed from pure chemical ingredient is called as
media.
Living Non living
Synthetic Complex
14) The osmotic pressure of culture media is adjusted by component.
NaCl Agar-Agar
Gelatin Yeast
2. Answer the following (any four) 8
Define enriched media. Give one example.
ii) Define semi synthetic media.
iii) Enlist types of objective lenses.
iv) DMC.
Define selective media.
Answer the following (any two) 6
Principle of fluorescent microscopy.
Principle of monochromatic staining.
Serial dilution technique.
3. Answer the following (any two) 10
Describe in brief principle and mechanism negative staining.
Discuss in detail tissue culture as living media.
Give significance of peptone, glucose, bile salt and lactose in culture
media.
Answer the following (any one) 4
Define stain. Describe in detail the types of stain with example.
Write note on principle and applications of dark field microscopy.
4. Answer the following (any two) 10
Give a detailed account on indirect methods of cell enumeration.
ii) Describe in detail phase contrast microscope. Add a note on its
applications.
iii) Define culture media. Describe in detail synthetic, natural and
enrichment media.
Answer the following (any one) 4
Discuss in brief principle and applications of electron microscope.
Discuss in short mechanism and procedure of acid fast staining.
5. Answer the following (any two) 14
Discuss in detail theories and mechanism of Gram staining.
ii) Describe in detail methods of pure culture techniques.
iii) Explain in detail principle, ray diagram and working of compound microscope.