B.Sc. (Biotechnology) (Part III) (Semester VI) (CGPA)
Examination, 2018
TOOLS AND TECHNIQUES
Day and Date Saturday, 31-3-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the rightside indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Rewrite the sentence using correct alternative given below 14
A rapid method of amplifying a length of target DNA is by
PCR Transformation
Labeling Transfection
Maxam Gilbert method is used for sequencing of
RNA DNA
Protein Other bimolecules
is a technique in which Minisatellite sequences are used for
analysis.
DNA fingerprinting Dot blot
Autoradiography DNA hybridization
A is a short or long length of ssRNA or DNA.
Nucleic acid Isotope
cDNA Probe
Restriction enzymes cleave the DNA at sites.
Nicks Single strand
Palindromic Ends
PBR322 is a
Cosmid Natural plasmid
Constructed plasmid Phagemid
Taq DNA polymerase is a enzyme used in PCR.
Thermolabile Thermostable
Halophilic Halophobic
In blotting techniques nucleic acids are transferred to the
membrane.
Cell Cellulose
Nitrocellulose None of these
The Crown Gall disease caused in plants is due to
Archaebacterium Agrobacterium Tumefacians
Agrobacterium rhizogenes Aerogenes
10) The technique of using electric current to allow entry of DNA into a cell is
called
Electrophoresis Electroporation
Microinjection Macroinjection
11) C-DNA library is prepared from
r-RNA t-RNA m-RNA Sn-RNA
12) is a self replicating entity used as a vector in gene transfer
technique.
Plasmid Virus Bacteriophage Probe
13) Insert size of 400 -450 Kb can be incorporated in a
Cosmid Virus Plasmid YAC
14) Type-II restriction endonucleases are most commonly used in
technique.
PCR RFLP
Western blotting Ligation
2. Answer any seven of the following 14
What is electroporation
ii) Explain the method of screening using nucleic acid hybridization.
iii) Give the role of c DNA probes.
iv) Give the characteristics of a plant viruses as vectors.
Explain the role of chromogenic substrates for screening of recombinants.
vi) Explain the method of DNA Foot-Printing.
vii) Discuss the method of automated sequencing.
viii) Give the significance of ligase enzyme.
ix) Explain the principle of complementation of defined mutations.
3. Write the short answers (any two) 10
Describe the technique of RAPD. Write its application.
How is selection of the recombinant clones done
Write down the role of different enzymes in gene cloning.
Write a note on agrobacterium mediated gene transfer technique. 4
4. Write short notes on any two of the following 14
Write an account on chromosome walking.
Describe the method of Southern blotting.
Comment on methods used for labeling of probes.
5. Attempt any two of the following 14
Enlist the names of cloning vectors with explanation.
Explain in detail the insert size of DNA used in various artificial chromosomes.
Explain the mechanism and give applications of DOT Blot technique.