B.Sc. III Biotechnology (Semester VI) (CGPA) Examination, 2018
tools and techniques
Day and Date Tuesday, 30-10-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives 14
Migration rate under unit potential gradient is called as of ions.
Mobility Absorptivity
Resistivity Viscosity
In which technique pH gradient in gel is used for separation
IEF PAGE
SDS-PAGE Sedimentation
VNTRs are analysed by
Electrophoresis RFLP
DNA sequencing RNA blotting
method is known as method of DNA sequencing.
Haberlandts Sanger's
Harrisons Gilbert's
Polymerase Chain Reaction is also known as
People's Created Robot People's Carrier Reaction
People's Choice Reaction People's Choice Radiator
cloning vector has COS site of λ phage.
pBR322 Phagemid
Cosmid Plasmid
analysis has been successfully applied for epidemiology of Listera monocytogens.
Protein Blotting Spectroscopic
DNA Sequencing RAPD
DNA sequencing method is known as chemical degradation method.
Sanger's Smith's
Carrel's Maxam's and Gilbert's
Molecular weight of subunit of alkaline phosphatase is
69 Kd 60 Kd
54 Kd 120 Kd
10) Minisatellites are
Short non-coding repetitive sequences present through out the chromosome
30-40 bp sized short sequences within the gene
Short coding repetitive regions on eukaryotic chromosome
Short coding repetitive regions on prokaryotic chromosome
11) RT-PCR is the method that is used for
Analysis of DNA Analysis of mRNA expression
Analysis of Plasmid Amplification of genomic DNA
12) Which of the following therapies developed by rDNA techniques
HIV vaccine Hepatitis B vaccine
Malaria vaccine Vaccine against cold
13) YAC stands for
Yeast Automated Chromosome
Yeast Artificial Clone
Yeast Artificial Chromosome
Yeast Automated Clone
14) Size of the pBR322 is
4.1 kb 4.66 kb
4.362 kb 4.22 kb
2. Answer the following (any seven) 14
Write a note on scope of Genetic Engineering.
Define restriction endonucleases.
Give brief account on c-DNA probes.
Write a note on nucleic acid modifying enzymes.
Draw a neat and labeled diagram of shuttle vector.
Enlist the applications of RAPD.
Explain principle of autoradiography.
Explain cloning from genomic DNA.
Write a note on screening by nucleic acid probes.
3. Answer the following (any two) 10
Explain direct DNA transfer by using electroporation.
Describe bacteriophage vector.
Discuss agarose gel electrophoresis for DNA.
Explain in short DNA transfer by Transduction. 4
4. Answer the following (any two) 14
Describe in detail AFLP as molecular marker.
Give details of RNA polymerases.
Discuss in detail chromosome walking.
5. Answer the following (any two) 14
Give details of automated DNA sequencing.
Discuss PCR and enlist its applications.
Explain yeast vectors.