B.Sc. Biotechnology (Semester (New CBCS) Examination, 2018
Tools and techniques
Day and Date Thursday, 22-11-2018 Total Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 14
Which is the technique suited for the separation of larger DNA fragments
PFGE AGE PAGE SDS-PAGE
When is electrophoresis not used
Separation of proteins Separation of lipids
Separation of amino acids Separation of nucleic acids
Which of the following factors does not influence electrophoretic mobility
Molecular weight Shape of molecule
Stereochemistry of molecule Size of molecule
Which of the following statements is correct with respect to exonuclease
They only act on single stranded DNA molecules
They only act on double stranded DNA molecules
They remove nucleotide bases from the middle of polynucleotide chain
They remove a single nucleotide base at a time
Which of the following statements is correct regarding S1 nuclease
It acts on single stranded DNA It acts on double stranded DNA
It acts on both types of strands It is obtained from E.coli
Type II cuts the sequence in the following way.
At 100-1000 nucleotides away from the recognition sequence
Within the recognition sequence
At 27-30 nucleotides away from the recognition sequence
It cuts randomly
The most popular and widely used engineered plasmid vector is
pSC 101 pUC 18
pBR 322 pBR 327
Autonomously Replicating Sequences is characteristic feature of
Plasmid vector Cosmid vector
Phage vector Yeast vector
Which of the following is not true about phagemids
Can only propagated as phage
Contain λ att site
Contain functional Ori of palsmid λ phage
May be propagated as a plasmid or as phage in appropriate strain
10) The ability of cells to take up DNA fragments from surrounding is called
Transduction Transformation
Transfection Conjugation
11) Which of the following chemical enhances vir gene expression
Cyanidin Glutennin
Acetosyringone Dextran
12) PCR is used in
Site specific recombination Site specific translocation
Site specific transformation Site directed mutagenesis
13) DNA footprinting is a suitable technique for identifying
Protein binding site within DNA Introns within DNA
tRNA associated with DNA mRNA associated with DNA
14) Alec Jeffery's name is associated with
DNA sequencing DNA fingerprinting
RNA sequencing Protein fingerprinting
2. Answer the following (any four) 8
Give brief account on introduction of genetic engineering.
Define exonucleases and endonucleases.
Define Vectors.
Enlist the advantages and applications of electroporation.
Define probes.
Write notes on (any two) 6
Write a note on transformation.
Write a note on particle gun mediated gene transfer.
Write a note on synthetic oligonucleotide probes and riboprobes.
3. Answer the following (any two) 8
Give details of agarose gel electrophoresis.
Explain AMV reverse transcriptase.
Discuss shuttle vector.
Answer the following (any one) 6
Describe chain termination method of DNA sequencing.
Discuss DNA blotting technique.
4. Answer the following (any two) 10
Explain SDS-PAGE technique.
Discuss reverse transcriptase PCR.
Describe chemical methods used for direct DNA transfer.
Answer the following (any one) 4
Describe DNA polymerase I.
Explain nick translation and random primed method of labeling.
5. Answer the following (any two) 14
Describe in detail RFLP.
Discuss in detail DNAse I and Ribonuclease H.
Explain pBR 322 and pSC 101 vector.