B.Pharmacy (Semester Examination, 2018
biotechnology (CGPA pattern)
Day and Date Thursday, 17-5-2018 Max. Marks 70
Time 10.30 a.m. to 1.30 p.m.
Note Figures to the right indicate marks.
1. Multiple Choice Questions (1×15=15)
proposed an idea that separate X and Y chromosomes determine sex.
Louis Pasteur Fleming
Wilson and Stevens Earnst Seyler
Protoplast viability can be determined by using dye.
Fluorescein diacetate Safranine
Crystal violet Congo red
developed BCG vaccine in 1908.
Chain Weizmann Selman Waksman
William Kolhe Calmette and Guerin
Identify the purine base present in DNA.
Adenine Cytosine
Guanine Both and
Sparger is used in fermentation for addition of
Antifoaming agent Antimicrobial agents
Sterile air Sterile medium
Agrobacterium tumefaciens is soil borne bacteria.
Gram positive Gram negative Both and None of these
Identify the strains used for commercial production of vitamin B12.
Streptomyces olivaceus Streptomyces griseus
Streptomyces albidoflavus All of these
An enzyme that cleaves DNA at specific site is called
Restriction ribonuclease Restriction endonuclease
Trypsin E.Coli DNA ligase
does not require aeration during their production by fermentation technology.
Penicillin Streptomycin Dextran Cobalamine
10) PCR is useful in the diagnosis of
HIV Fever Diabetes None of these
11) Northern blotting technique is used for the confirmation of
DNA RNA
Both DNA and RNA Proteins
12) Identify an enzyme not to be used in disaggregation of tissues in animal cell culture.
Catalase Trypsin Collagenase Pronase
13) is an example of cytokinine.
Kinetin Zeatin
6-benzyl amino purine All of these
14) extract is most commonly used for growth of animal cell and it's substituted by mixture of amino acids.
Chick embryo Liver Spleen Bone marrow
15) is mainly used for cell division and root initiation in cultured tissues.
Gibberellin Cytokinin Abscisic acid Auxin
2. Answer any five of the following questions
Discuss future scope of biotechnology in pharmaceutical industry.
Explain construction of conventional fermenter with neat labelled diagram.
Write a note on cryopreservation technique with their applications.
Add a note on electroporation with their merits and demerits.
Explain plasmid as a vector.
Describe artificial media used in animal tissue culture.
3. Answer any three of the following questions (3×10=30)
Explain in detail different bioconversion reactions with special reference to steroids.
Discuss in detail requirements for establishing tissue culture laboratory.
Describe any two blotting techniques with their applications.
Discuss insulin production by r-DNA technology.