Exam Details

Subject plant biology biotechnology
Paper
Exam / Course m.sc
Department
Organization central university
Position
Exam Date 2016
City, State telangana, hyderabad


Question Paper

1. If a gene has a different effect depending on whether it is inherited maternally or paternally, this is called

DNA

incomplete dominance

imprinting

karyotype



2. Which molecule in the nucleus controls the copying of DNA?

carcmogen

protein kinase

polymerase

cyelin

3. What chemical do plants absorb through the roots to allow them to make proteins?

nitrate

phosphate

zinc

uranium

4. In which direction do the sodium and potassium ions move in the sodium/potassium pump?

Na+ into the cell, out

into the cell, Na+ out

Na+ into the cell, in

out of the cell, Na+ out

5. Which one of the following plants does not have xylem?

Pinus radiata

Pisum sativum

Pteris vittata

Marchantia polymorpha

6. Which among the following reagents is used to distinguish between phenol and benzoic acid?

Neutral FeCh

Aqueous NaOH

Tollen's reagent

Molisch reagent

7. The polar transport is characteristic of the plant hormone

Abscisic acid

Gibberellic acid

Indole acetic acid

Jasmonic acid

8. Darwin showed that all species can rapidly increase in population, but most wild populations are __over time.

decreasing

stable .

increasing

none of the above

9. Zygotic meiosis is a characteristic feature of

Algae

Pteridophytes

Gymnosperms

Bryophytes

10. Death of protoplasm is a pre-requisite for a vital function like

transport of sap

absorption of water

gaseous exchange

transport of food

11. A vybrid is the progeny obtained from a cross between

two hybrid varieties

two facultative apomicts

two obligate apomicts

two somatic hybrids

12. Two cells A and B are contiguous. Cell A has osmotic pressure 10 atm, turgor pressure 7 atm and diffusion pressure deficit of 3 atm. Cell B has osmotic pressure 8 atm, turgor pressure 3 atm and diffusion pressure deficit of 5 atm. The result will be

no movement of water

equilibrium between the two cells

movement of water from cell A to B

movement of water from cell B to A

13. One set of the plants were grown at 12 hours day and 12 hours night period cycles and they flowered while in the other set, night phase was interrupted by a flash of light and the plants did not produce flowers. Under which one of the following categories will you place this plant?

long day

day neutral

darkness neutral

short day

14. What is a spheroplast?

It refers to a bacterium or a plant cell bound by its plasma membrane, the cell wall being deficient or lacking and the whole having a spherical form

It refers to a bacterium or a plant cell bound by its plasma membrane, along with the cell wall and the whole having a spherical form

It refers to plant cell organelles which are having a spherical form

It refers to a bacterium or a plant cell without plasma membrane, having only cell wall and the whole having a spherical form

15. In a non-dividing cell, a complex ofDNA and proteins would be

ribpsomes

chromatin

codons

anticodons

16. Glyphosate is a systemic herbicide which acts by inhibition of

Enolpyruvate shikimate-3-phosphate synthase of the aromatic amino acid biosynthetic pathway

Ribulose 1,5-bisphosphate carboxylase of the Calvin cycle

Chorismate synthase of the aromatic amino acid biosynthetic pathway

Phosphoglycerate kinase of the Calvin cycle

17. Why do restriction enzymes not destroy the DNAofthe organisms that make them?

Modification enzymes inactivate the restriction enzymes until needed

Their genomes do not contain the restriction site that their own enzymes recognize

Restriction enzymes are secreted and are not found in the producer cells

Modification enzymes protect their DNA by methylation

18. PCR can be used to synthesize RNA provided that

RNA polymerase is used instead of Taq polymerase

Both RNA polymerase and Taq polymerase are used

Ribonucleoside triphosphates are used instead ofdNTP's

None of the above allow RNA to be made by peR

19. The number of cells present in angiosperm embryo sac are

five

six

seven

eight

20. Why are plants more readily manipulated by genetic engineering than animals?

plant genes do not-contain introns .

more vectors are available for transferring recombinant DNA into plant cells

a somatic plant cell can often give rise to a complete plant

genes can be inserted into plant cells by microinjection

21. Which of the following have no enzymes of their own?

bacteria

viruses

cyanobacteria

all of these

22. All of the following karyotypes are found in spontaneous abortions. Which of the following is least likely to be found in a live-born infant?

23. An organism with two or more different cell lines originating from a single zygote is called a

Syndrome

Chimera

Mosaic

Heterozygote

24. Kinase reactions

inhibit ATP breakdown

involve the addition of a phosphate group

involve the addition or removal of an amino acid to a polypeptide chain

involve the transfer of hydrogen atoms

25. Complete the following statement correctly: FAD is a prosthetic group, .....



45, X



47, XX, +16 a it is readily exchanged with the solvent. b it is loosely associated with the enzyme. c .it is an artificial substitute for NADH. d it is tightly bound to the enzyme.

26. What is the other name for the chromosome that defines the genetic gender of an individual?

gonosome

autosome

karyotype

allosome

27. The amount of water lost by transpiration from plant is:









28. Which of the following metal ion is part of plastocyanin molecule?

Copper

Manganese

Magnesium

Aluminum

29. Two solutions of a substance (non-electrolyte) are mixed in the following manner: 480 mL of 1.5 M first solution 520 mL of 1.2 M second solution. What is the molarity of the final mixture?

1.20 M

1.344 M

(c)1.50M

(d)2.70M

30. In which of the following crosses in pea plant, 1 phenotypic ratio will be obtained:

TTRR ttrr

TtRr ttrr

TtRr Ttrr

TtRr TtRr

31. The transgenic rice with high carotenoids level is popularly called

Orange rice

Red rice

Golden rice

Yellow rice

32. A man heterozygous for the gene causing haemolytic jaundice marries a normal woman for this gene. The disease is due to dominant gene but only 10% of the individuals having genotype that can cause disease actually develop disease. What proportion of the children of the above couple have the probability to have the disease?

1/20

1/5

1/10



33. Which of the following statements about allosteric control of enzymatic activity is false?

Allosteric effectors give rise to sigmoidal Vo vs kinetic/plots

Allosteric proteins are generally composed of several subunits

An effector may either inhibit or activate an enzyme

Heterotropic allosteric effectors compete with substrate for binding sites

34. The energy charge of the cell is:

the difference between the charge on the outside and inside of a cell

generated by the sodium-potassium ATPase .

the overall rate of energy use by the cell

the extent to which the total adenine nucleotide pool is phosphorylated

35. In X chromosome inactivation, all of the X chromosome genes are inactivate(d)

true

false

not sure

impossible

36. A lod score refers to:

locus ofDNA .

log odds

location ofDNA

mRNA

37. The basis of precipitation of proteins by ammonium sulfate is best described by which of the following statements?

Proteins become insoluble when they bind the ammonium ion

Proteins become insoluble when they bind sulfate ion

Addition of ammonium sulfate adjusts the pH to the isoelectric point of the proteins

Ammonium sulfate binds water molecules, making them less available for hydration of proteins

38. Cell division cannot be stopped in which phase of the cell cycle?

Gl phase

G2 phase

S phase

Prophase

39. Plants like Aegle mannelos, Ocimum sanctum and Ficus religiosa are a group of plants designated as

Traditional food crops

Sacred species

Medicinal Plants

Lesser known food plants

40. Measuring the absorption of ultra violet radiation at 260 nm can be used to assay the concentration ofDNA and RNA because

The base of each nucleotide absorbs at 260 nm

The phosphate group of each nucleotide absorbs at 260 nm

The pentose sugar of each nucleotide absorbs at 260 nm

None of the above is correct

41. Subject that deals with factors that affect the earth and air pollution is termed as

Dendroclimatology

Climate Change

Dendrohydrology

Dendrochronology

42. Chromosomes which replicate using multiple origins are

Mammalian

Bacterial

Prokaryotic

Viral

43. Which is correct about conduction of substances?

organic food moves up through phloem

organic food moves up through xylem

inorganic food moves upwardly and downwardly through xylem

organic food moves upwardly and downwardly through phloem

44. Topoisomerases are enzymes with

Both polymerization and unwinding activity

Both nuclease and ligase activity

Both transcription and ligase activity

Both polymerization and nuclease activity

45. A common structural feature of sieve tube elements and vessel elements is

pores on lateral walls

enucleate condition

thick secondary walls

presence ofP protein

46. Movement of water through semipermeable membrane produces

wall pressure

turgor pressure

osmotic pressure

suction pressure

47. Acid concentration ofCAM plants is more at

dawn

dusk

night

(d)day

48. Cell elongation in internodal regions of green plants occurs due to the action of

gibberellins

auxins

ethylene

cytokinins

49. In tissue culture, a low cytokinin to auxin ratio causes

Root differentiation

Flower differentiatioh

Shoot differentiation

Tuber formation

50. Which among the following is a mismatch?

Osmotrophs -Dissolved organic/inorganic nutrients

Phagotrophs -Feed by engulfing

Mixotrophs -Organic and inorganic carbon as nutrient source

Oligotrophs -Nutrient rich

51. Find out the odd one among the following:

Lactose, Sucrose, Maltose

Glucose, Fructose, Talose

Ribose, Xylose, Arabinose

Galactose, Talose, Lyxose

52. Plant conservation day is celebrated every year on

May 18th

June 5th

August 21st

September 30th

53. Lithotrophs use inorganic compounds as

Carbon source

Nitrogen source

Electron donors

Energy source

54. Which among the following is not a component of bacterial cell wall?

Mycolic acid

D-Amino acids

Sugars

Terpenoids

55. This bacterium causes tumour formation on a wide variety of plant species and orders the plant to proliferate its cells and makes food for itself. This bacterium also helps in genetic transformation of crop plants.

Azotobacter chroococcum

Xanthomonas oryza

Bacillus thuringiensis

Agrobacterium tumefaciens

56. Identify the mismatch

Negative staining -Klebsiella

Acid-fast staining -Mycobacterium

Gram staining -Clostidium

Malachite green -Escherichia

57. Which of the following acids does not exhibit optical isomerism?

Lactic acid

Tartaric acid

Maleic acid

-ids

58. The water stress mediated stomatal closure in leaf is mediated by plant hormone

Salicylic acid

Gibberellic cid

Abscisic acid

Ethylene

59. If the molar amount ofG in a DNA sample is what is the molar amount ofT in the sample?









60. A gymnospermic leaf carries 16 chromosomes. The number of chromosomes in its endosperm will be



8

16

24

61. Term applied to plants having separate male and female plants is

Monoecious

Monogamous

Polygamous

Dioecious

62. An abandoned, idled, or polluted site is called

Whitefield

Blackfield

Brownfield

Redfield

63. Strutevant's detailed mapping studies of the X chromosome ofDrosophila established that .

genes are carried on chromosomes

the sex-determination is controlled by the X and Y chromosomes

the different pairs of chromosomes assort independently

genes are arranged in a linear order on the chromosome

64. Dimethyl benzene is also known as

Toluene

Cumene

Xylene

Styrene

65. Glycine is a precursor of

Proteins

Lipids

Porphyrins

Carbohydrates

66. Pick the correct electron transfer route the light-dependent reactions in plants.

P680 -H2O -P700 -NADP+

P700 -NADP+ -H20 -P680

H2O -P680 -NADP+ -P700

H2O -P680-P700-NADP+

67. In Drosophila melanogaster, singed bristles and cut mgs are both caused by recessive, X-linked alleles. The wild type alleles sn+ and are responsible for straight bristles and intact wings, respectivel . A female homozygous for sn and ct+ is crossed to a sn+ct male. The Fl flies are interbre(d) The males are distributed as follows:

sn ct 14

sn ct+ 34

sn+ ct 37 sn+ ct+ 15

What is the map distance between sn and ct?

14.5 map units

29 map units.

35.5 map units

24 map units

68. The cell that undergoes programmed cell death to become functional is

phloem sieve tube member

xylem vessel member

stomatal guard cell

root cap cell

69. The best approach for preventing over ripening of banana is

putting them in a bag or in a cup board

exposing them to heat

dipping them in ascorbic acid

by keeping them with other ripened fruits at room temperature

70. Which of the following statements about Mendel's breeding experiments is incorrect?

the parental plants were true breeding

All of the F1 progeny resembled one of the parental plants, but only some of the F2 progeny did

Half of the F1 progeny had the same phenotype as one of the parental plants, and the other half had the same phenotype as the other parent

the traits that disappear in the F1 reappear in the F2 generation

71. A pedigree chart shows

the genotypic ratios of the offspring

the types of gametes produced by the parents

the genotypes of any parents

the mode of inheritance of a specific gene

72. Man dominated ecosystem is called

Geosystem

Biome

Noosystem

Community

73. Vibrio cholerae has

Monotrichous flagella

Bip. r single flagella

Peritrichous flagella

Lophotrichous flagella

74. Which one among the following is correct for IMViC (Indole; Methyl red; Voges­Proskauer; Citrate) test for Klebsiella pneumonia

MR VP Indole -ve

MR VP Indole +ve

MR VP Indole +ve

MR VP Indole -ve

75. In plants, inulin and pectin are

reserve material

wastes

excretory material

insect attracting material

76. If the whole chain is used in a non-overlapping frame, how many amino acids are defined by this DNA sequence: ATGTTTGGACTA?

Three

Four

Six

Twelve

77. One of the organic compounds is frequently used in several molecular biology and biochemistry experiments. This organic compound is prepared in 3 steps in the laboratory. This compound is prepared by heating ethyl alcohol or acetone with bleaching powder. The bleaching powder acts as source of chlorine and calcium hydroxide. The finally formed organic compound is

TRIS-HCI

Chloroform

ß-mercaptoethanol

Polyethylene glycol

78. Grignard reagents are highly reactive and used in the synthesis of alkanes, alkenes, alcohols, and aldehydes, Which of the following compounds are Grignard reagents?

Methyl magnesium iodide and ethyl magnesium bromide

Methyl bromide and ethyl iodide

Lithiu' I copper and methyl lithium bromide

T acarbonyl nic I and diethyl zinc

79. What is the meaning of the term "Sanguivorous"?

Parasite which fee on the blood of cattle

Parasite which grows in saliva of mammals

Parasite grows on plant roots present on heavy metal polluted area

These are animals which can sustain extreme high or cold temperatures

80. This compound/gas produces painful blisters on the skin and has very destructive effect on the lungs and air passages. This compound/gas is prepared by treating ethylene with sulphur monochloride. This compound/gas is

Phosgene

Ethyl isocyanate

Cyanogen Chloride gas

Mustard gas

81. Lectin is a

Protein

Lipid

Monosaccharide

Polysaccharide

82. The genus Laminaria that is used for the manufacture of iodine belongs to

Algae

Fungi

Bryophytes

Pteridophytes

83. One of the following class Of compounds acts as an attractant for Rhizobium­mediated legume root nodulation

Alkaloids

Flavonoids

Terpenoids

Carbohydrates

84. A research scholar isolated genomic DNA from rice leaf in the lab and he checked the quality and quantity of DNA using agarose gel electrophoresis. Further, he took a certain amount of DNA in three different tubes and added BamHI, HindIII and NotI separately. After few hours of incubation at required temperature, he again checked the final product using agarose gel electrophoresis. What are BamHI, HindIII and NotI?

BamHI and HindIII are restriction endonucleases; NotI is the name ofDNA probe which is responsible for detection of conserved sequence

HindIII and NotI are restriction endonucleases; BamHI is a protein denaturing agent isolated from Bamboo plant

BamHI is a protein denaturing agent isolated from Bamboo plant; HindIII is a chemical which is used for DNA cleaning and purification; NotI is chemical which does not allow RNA or cell debris to stay with DNA.

BamHI, HindIII and NotI are all restriction endonucleases

85. The female sex organ of the bryophytes is called

Antheridium

Archegonium

Phycobiont

Storobili

86. The biological role of restriction enzymes in bacteria is to

Repair DNA

Induce DNA crossover

Cleave foreign DNA

None of the above

87. When carboxylic acids react with alcohols in the presence of a strong catalyst like H2S04 or HCI, this leads to formation of .

Amide

Aldehyde and ketone

Hydroxy and halo acids

Esters

88. Which of the following is known as Hinsberg reagent?

Benzene sulphonyl chloride

Thiophenol

Oxalyl Chloride

Phosphoryl chloride

89. The following structure of an organic acid is found naturally in many plant species. What is the name of this organic acid?

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Tartaric acid

Citric acid

Glucuronic acid

Gluconic acid

90. Which of the following eukaryotic cell wall components are nitrogenous compounds?

lignin

cellulose

chitin

ß-glucan

91. A special pigment complex found in cyanobacteria is called

phycobilisome

phycoerythrine

light harvesting complex

Phytochrome

92. Citric acid cycle occurs in

grana

cytoplasm

cristae

mitochondrial matrix

93. alpha-helix has

304 amino acid residues/tum

(b)3.0 amino acid residues/turn

3.6 amino acid residues/turn

3.8 amino acid residues/turn

94. The enzyme Dehydrogenase can be classified as

Oxidoreductase

Transferase

Hydrolase

Ligase

95. A silent mutation is most likely to result from

substitution of the first base of a codon

substitution of the third base of a codon

conversion of a nonsense codon into a sense codon

conversion of a sense codon into a nonsense codon

96. Which genus of bacteria listed below grows best at 110°C?

Sulfolobus

Halobacterium

Methanosarcina

Escherichia

97. If a trait has a heritability of 60%,how much of the variation is caused by genes?









98. A mouse eats plant seeds. If the mouse is eaten by a snake, and the snake in turn is eaten by an eagle, the eagle is a

producer

primary consumer

secondary consumer

tertiary consumer

99" Match items in Column I with those in Column II:

Column I Column II

A. Peritrichous flagellation J. Ginkgo

B. Living fossil K. Macrocystis

C. Rhizophore L. Escherichia coli

D. Smallest flowering plant M. Selaginella

E. Largest perennial alga N. Wolffia

Select the correct answer from the following.

E-K

E-N

E-J

E-K

100. Which of the following micro-organisms is used for production of citric acid in industries?

Aspergillus niger

Rhizopus nigricans

Lactobacillus bulgaris

Penicillium citrinum


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