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ENTRANCE EXAMINATION-2010 M.Sc. Plant Biology and Biotechnology 05-06-2010
Time: 2 hours Maximum Marks: 100
HALL TICKET NO.

INSTRUCTIONS Please read carefully before answering the questions:
1.
Enter your Hall Ticket number both on the top of this page and on the OMR answer sheet.

2.
Answers are to be marked only on the OMR answer sheet following the instructions provided there upon.

3.
Hand over both the question paper booklet and OMR answer sheet at the end ofthe examination.

4.
The question paper contains 100 questions Question Nos. 1-25 and Part-B: Question Nos. 26-100) ofmultiple choice typed in 19 pages, including this page. One OMR answer sheet is provided separately. Please check.

5.
The marks obtained in Part-A will be used for resolving the tie cases.

6.
Each question carries one mark.

7.
There is negative marking for wrong answers, in PARTS A and B. For each wrong answer, 0.33 ofa mark will be deducted.

8.
Calculators and mobile phones are not allowed.




PART-A

1. Science that involves the extraction and analysis of the DNA from all
microorganisms living in a particular environmental sample without the need to culture them is
A. Genomics B. Eugenomics
C. Hologenomics D. Metagenomics
2. Which of the following plant groups lacks lignified cell walls in its cellular structure?
A. Bryophytes B. Halophytes
C. Pteridophytes D. Spermatophytes
3. When 20% ofchiasma are formed between two loci, A and B among the
tetrads of an individual of the genotype AB/ab, the percent of gametes expected to be are
A. 5
B. 10
C.20
D.40
4. A common symptom in plants caused by the deficiency ofP, Ca and Mgis
A. The formation ofanthocyanins
B. The bending ofleaftop
C. The poor vasculature
D. The development ofnecrotic areas
5. The role of a "Flag leaf' is to
A. provide nourishment to the growing inflorescence
B. enhance the nitrogen fixation
C. prevent the stomatal opening
D. provide a means ofvegetative reproduction
6. Polynucleotide ligase joins two DNA molecules together by forming a covalent bond between
A. two OH groups ofadjacent strands
B. a 3'OH group and a 5'P04 group
C. a 3'P04 group and a 5'OH group
D. two carbon atoms ofadjacent nucleotides on the same strand
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7. Oneoftherudimentaryleavesthatprecedeastage ofgrowth
A. Protophyll B. Cataphyll
C. Stipule D. Primary leaf
8. The correct sequence oftaxonomic categories is
A. phylum -class -family -order -genus -species
B. phylum -class -order -family -genus -species
C. phylum -order -class -family -genus -species
D. class -phylum -order -family -genus -species
9. Which of the following is a differential staining that is preferably used by microbiologists to identify pathogens whose cell walls are known to have high lipid content
A. Gram staining B. Acid Fast staining
C. Negative staining D. Positive staining
10. Match the parasitic plants listed in the left panel with the type of parasitism from the right panel and choose the correct answer
Name ofthe parasitic plant Type of the parasitism
L. Santalum album I. Total root parasite
M. Orobanche
II. Partial stem parasite
N. Loranthus
III. Total stem parasite
O. Cuscuta IV. Partial root parasite
A. N-III; O-II
B. L-III; O-II
C. 0-111
D. M-III; 0-1
11. Identify the INCORRECT statement about ABO blood type alleles in humans
A. It has three common alleles
B. Type 0 Blood can be used in transfusion for individuals of any blood type
C. It controls the type of glycolipids found on the surface of erythrocytes
D. Individuals with blood type AB have anti-A and anti-B antibodies in their blood serum
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12. In 1933, Hitler became the chancellor of Gennany and the same year a major invention changed the face of microbiology. Select the closest landmark discovery, among the following, that had a great impact on
development ofmicrobiology.
A. Walksman discovered streptomycin
B. Felming discovered penicillin
C. Ruska developed electron microscope
D. Griffith discovered bacterial transfonnation
13. Which ofthe following is NOT TRUE with regard to imprinted genes?
A. They provide an example ofepigenetic inheritance
B. Are near differentially methylated regions
C. Their expression is detennined by the parent that contributes them
D. They are heritable phenotypes in which the genotype plays an important role
14. Rubisco, the most abundant protein present on earth is part of
A. Calvin cycle B. Glycolysis
C. Krebs cycle D. Cell cycle
15. Ficus elastica, the latex of which is used in the manufacture of Indian rubber, belongs to the family
A. Moraceae B. Asteraceae
C. Apocynaceae D. Meliaceae
16. One of the research scholars has isolated a novel protein from a medicinal plant which enhances the immunity in humans. After isolation, he purified the protein by a protein purification method in which he used Sephadex G-I00. Which technique he has used for the purification ofnovel protein?
A. Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis
B. Pulse Field Gel Electrophoresis
C. Counter Immuno Electrophoresis
D. Gel Filtration Chromatography
17. The source of Cry1Ac gene which has been used for engineering resistance to lepidopteran insects in cotton is
A. Bacillus subtilis B. Bacillus amyloliquefaciens
C. Bacillus thuringiensis D. Bacillus circulans
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18. Ammonia oxidation to nitrate depends on the following two bacteria
A. Nitrosomonas-Nitrosospira
B. Nitrobacter-Nitrococcus
C. Nitrosospira-Nitrococcus
D. Azospirillum-Pseudomonas
19. Which ofthe following pairs is correctly matched? chain fatty acids and polyhydroxy

B. Pectin: Methylated polymer ofgalacturonic acid
C. Lignin: Formed by condensation of coniferyl alcohol
D. Callose: Glycosylated aliphatic acid
20. The 2009 Nobel Prize in Chemistry was awarded to Ada E. Yonath, Thomas A. Steitz and Venkatraman Ramakrishnan for
A. studies ofthe structure and function ofthe ribosome
B. discovery of"Split gene"
C. discovery oftelomers and telomerase
D. discovery of RNA interference -gene silencing by double-stranded RNA
21. Which of the following was used for the 'Green Revolution' in the country?
A. Basmati rice B. Dwarfvariety ofwheat C. Hybrid sugarcane D. C S H-5 Jowar
22. The drug chloramphenicol blocks
A. cell wall formation B. transcription
C. polypeptide chain initiation D. polypeptide chain elongation
23. Which of the following is the best evidence for template theory of enzyme action?
A. Compounds similar in structure to the substrate inhibit the reaction
B. Enzymes speed up the reaction by definite amounts
C. Enzymes are found in living organisms and increase the rate of certain reactions
D. Enzymes determine the rate of a reaction
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24. Match the common names of the plants listed in the left panel with their scientific names from the right panel choose the correct answer.
Common name Scientific name
L. Finger millet I. Avena sativa L
M. Foxtail millet II. Pennisetum glaucum L.
N. Pearl millet III. Eleusine coracana L.
O. Oat IV. Setaria italica L.
V. Secale cereale L.
A. 0-1
B. L-III; 0-1
C. L-III; a-I
D. a-III
25. There are different geographic modes of speciation in nature, based on the extent to which speciating populations are geographically isolated from one another. Identify the description from the Column-B that matches the different forms of speciation given in the Column-A and mark the correct answer
Column-A Column-B
L. Allopatric
I. Organisms whose geographical ranges do not significantly overlap but are immediately adjacent to each other.
M. Peripatric II. Organisms whose geographical ranges overlap or are even identical, so that they occur together at least in some places.
N. Parapatric III. Organisms whose geographical ranges are closely adjacent but do not overlap, being separated by a natural barrier.
O. Sympatric
IV. Organisms whose geographical ranges are entirely separate, so that they do not occur in anyone place together.
A. M-III; 0-1
B. M-III; a-II
C. L-III; a-I
D. L-III; a-IV
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26. Copper, an essential micronutrient, is present in which of the following molecule
A. Plastoquinone B. ChlorophyllC. Plastocyanin
D. Thioredoxin
27. In the absence of a transilluminator, RNA in an agarose gel can be visualized with a naked eye by this staining
A. Coomassie brilliant blue R 250 B. Crystal violet C. Ethidium bromide
D. Toluidine blue
28. All of the following features enable the red algae to grow to greater depths than brown and green algae EXCEPT
A. Carotenoids B. PhycobilinsC. Chlorophyll a and c D. Laminarins
29. The sequence of a protein can be determined using several methods.
The preferred method, which labels and releases the N-terminal residue,is called
A. Sanger method B. Edman degradation C. CNBr cleavage D. Ninhydrin method
30. All ofthe following are examples oflipids EXCEPT
A. phospholipids B. chitin
C. waxes
D. cholesterol
31. The leaf powder of the following plant is commonly used for treating diabetes.
A. Azadirachta indica B. Ocimum sanctum
C. Terminalia arjuna D. Gymnema sylvestre
32. Fragmentation of filamentous cyanobacteria can generate small, motile fragments called
A. Akinetes
B. HormogoniaC. Trichomes D. Heterocysts
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33. The following compounds are isomers
A. ethanol and dimethyl ether B. ethanol and acetone
C. acetic acid and oxalic acid D. ethanol and acetic acid
34. Match the phenomena listed under the left panel with the ratio of epistasis given in right panel and choose the right option
Type of epistasis Ratio of epistasis
L Dominant epistasis I. 7
M. Recessive epistasis II. 12:3:4
N. Complementary epistasis III. 13:3
O. Inhibitory epistasis IV.
A. M-III; 0-1 B. L-III; 0-1
C. O-III D. 0-111
35. Evidence indicating that chloroplasts were originally free living prokaryotes that subsequently evolved a symbiotic relationship with a eukaryotic host includes all ofthe following EXCEPT the
A. similarities of rRNA sequences between chloroplasts and free-living prokaryotes
B. similarities in structure between the chloroplasts and some contemporary free-living prokaryotes
C. ability ofchloroplasts to synthesize all their own proteins
D. presence of circular DNA in chloroplasts and in free-living prokaryotes
36. Gametophyte is least developed in
A. bryophytes B. pteridophytes
C. algae D. fungi
37. Role ofmycorrhiza is to increase
A. phosphorous availability B. potash availability
C. nitrogen availability D. calcium availability
38. Thermophiles have temperature optima between
A. 45-50°C B. 55-65°C
C. 70-85°C D. 80-90°C
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39. Endospores are
A. certain fungal spores, enable their speCIes to survive in adverse conditions
B. certain bacterial spores, enable them to survive in adverse conditions
C. certain protozoan fruiting bodies, enable them to survive in adverse conditions
D. non-living viral capsules, capable ofinfecting eukaryotic cells
40. A graduate student had to investigate the light intensity dependant changes in the PEP carboxylase enzyme levels in the leaves of Amaranthus
Thf"Tf"fnTf" thp l;1tnr1pnt 01'PUT tho AM1,.,..,....1'£.....
.•_.... ----......... ....-.... b.A._... .t.A.I'.....'

hypochondriacus plants and exposed fully expanded leaves to series of light intensities medium and high) and prepared the samples for an appropriate assay. Which of the following techniques should be used by the student to investigate light dependent changes in the PEP carboxylase
enzyme?
A. Transformation B. Northern blotting
C. Immuno blotting D. Southern blotting
41. Which of the following is NOT TRUE with regard to replication cycles used in Polymerase Chain Reaction
A. 90-96°C -DNA is denatured into single strands
B. 50-60°C -The primers anneal to their complementary sequences
C. 72°C - The polymerase binds and extends a complementary DNA strand from each primer
D. 80°C - The polymerase adds dNTP's from reading the
template from

42. Which of the following characteristic is UNDESIRABLE in a cloning vector?
A. Control their own replication
B. High copy number
C. Small in size
D. Vulnerable at several sites to a restriction enzyme
43. The assimilatory power in plants refers to
A. splitting ofwater
B. reduction of CO2
C. production of ATP and NADPH
D. production ofNADH and Oxygen
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44. Select the scientist who has discovered that soil bacteria could oxidize iron, sulfur and ammonia to obtain energy
A. Elie Metchinkoff B. Sergei Wingradsky
C. Martinus Beijerinck D. Emil von Bhering
45. Match the type of bond with their role below and choose the correct answer
Bond type
L. phosphodiester 1. links base to pentose in nucleotide
M. N-glycosidic II. joins adjacent nucleotides in one strand
N. phosphate ester III. joins complementary nucleotides in two strands
O. hydrogen IV. difference between a nucleoside and nucleotide
A. O-III
B. O-III
C. L-III; O-II D. O-III
46. Which of the following plants is used as a model system for studies on somatic embryogenesis?
A. Zea mays L. B. Nicotiana tabacum L.
C. Daucus carota L. D. Gossypium hirsutum L.
47. Which of the following IS NOT an intermediate of the TCA (Tricarboxylic acid) cycle?
A. Malate B. Succinate
C. Citrate D. Fumarate
48. Which ofthe following acts as a tag to lysosomal enzymes?
A. Mannose-6-phosphate B. Pentose-6-phosphate
C. Fructose-6-phosphate D. Glucose-6-phosphate
49. Which one of the following reactions, cycles, or pathways is NOT found in plant systems?
A. The urea cycle
B. The glyoxalate cycle
C. The rubisco reaction
D. The gluconeogenesis pathway
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50. Which of the following statements IS INCORRECT about sex determination in Drosophila?
A. The flies with a Y chromosome but no X chromosome do not survive
B. The female determiners are located on the X-chromosomes and male determiners on the autosomes
C. The XO flies are sterile males
D. The combination of two X chromosomes and two sets of autosomes produces a normal diploid female
51. The Scientific names of medicinal plants are given in Column-A.
Identify the family to which they belong from the Column-B and mark the correct answer.
Column-A Column-B
L. Hemidesmus indicus
I. Lamiaceae
M. Ocimum sanctum
II. Asclepiadaceae
N. Rauvolfia serpentina III. Rubiaceae
O. Centella asiatica IV. Apocynaceae
V. Apiaceae
A. O-III
B. O-V
C. L-III; O-II
D. M-III; O-V
52. The high-efficiency particulate air (HEPA) filters used in laminar flow chambers can filter particles up to
A. 0.01 f.!m B. 0.05 f.!m
C. 0.2 f.!m D. 0.3 f.!m
53. Which among the following support lithoautotrophic growth of microorganisms?
A. H2S C02 B. H2S glucose
C. Glucose CO2 D. CO2 H2S
54. Breaking the seed dormancy by low temperature treatment is called
A. Scarification B. Stratification
C. Vernalization D. Lyophylization
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55. The assimilation of C02 into organic compounds (triose phosphates) in green plants:
A. results in the production of ATP
B. takes place at equal rates in light and darkness
C. requires NADPH
D. involves condensation ofthe two-carbon compound acetate with CO2 to form 3-phosphoglycerate
56. Water moves from the roots to the leaves of plants. In this regard, one ofthe following statements is NOT true?
A. water is pushed by solutes
B. capillary action pulls the water molecules like a chain
C. evaporation at the leaves pulls on the water
D. waters cohesion causes it to pull towards the leaves
57. Which of the following halogens are arranged in the order of electronegativity?
A. Br
B. CI> Br> I>F
C. Br>I
D. I
58. Polysomes are
A. complexes of endosomes
B. complexes oflysosomes
C. complexes offreeribosomesinthecytosol ofaneukaryoticcell
D. complexes of ribosomes bound together by a single mRNA
59. Which of the following releases most energy when completely oxidized in the body?
A. One gram ofglucose
B. One gram ofprotein
C. One gram ofalcohol
D. One gram ofpalmitic acid
60. C4 plants possess a characteristic leaf anatomy called "Kranz". Which ofthe following is missing in Kranz anatomy?
A. Bundle sheath B. Palisade mesophyll
C. Spongy mesophyll D. Stomata
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61. Gram negative pathogenic bacteria secrete important virulent factors using this secretion pathway
A. Tat pathway
B. Type V secretion pathway
C. Type VI secretion pathway
D. ABC pathway
62. Which ofthe following statements is NOT true about apomixis?
A. It is asexual reproduction through seeds without fertilization
B. In gametophytic apomixis, the embryo sac has the same number ofchromosomes as the mother plant
C. In sporophytic apomixis, an embryo is formed directly from nucellus or integument tissue
D. Apomictically produced offspring are genetically different from the parent plant
63. Match the biological term in the Column-A with the matching description in the Column-B and mark the correct answer
Column-A Column-B
L. Conjugation I. Plasmid gets integrated into chromosome.
M. Transformation
II. Process In which any gene may be transferred from one bacterial cell to another by a virus.
N. Generalized III. Bacterium takes up DNA from the transduction medium in which it is growing.
O. Specialized IV. Genetic material passes directly from one transduction bacterium to another.
V. Bacterial genes near the site of prophage insertion are transferred to another bacterium.
A. L-III; O-V
B. L-III; O-II
C. M-III; O-V
D. M-III; O-II
64. Oneofthefollowingis NOTamember ofEnterobacteriaceae
A. Serratia B. Shigella
C. Klebsiella D. Stigmatella
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65. The type of plumage found in mallard ducks is determined by three alleles at a single locus; MR, which encodes restricted plumage; which encodes mallard plumage; and md, which encodes dusky plumage. The restricted phenotype is -dominant over mallard and dusky; mallard is dominant over dusky M md). What is the expected phenotypes and proportions of offspring produced in the cross
MRMXMmd?
A. Yzrestricted, mallard, dusky
B. Yz restricted, Yz mallard
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v. /4 lU411aIU
D. restricted, mallard, 14 dusky
66. Promoter is
A. upstream RNA sequence of a mRNA, which is recognized by translation initiation factors in order to initiate translation
B. upstream DNA sequence of a gene, which is recognized by RNA polymerase in order to initiate transcription
C. sequence ofamino acids in a protein, which promote catalysis of an enzyme
D. sequence of amino acids in a protein, which specifically promote oxidative/reductive reactions
67. Counting of bacterial cell numbers has been with the help of an electronic device called coulter counter. In the recent years, an alternative and more precise instrument has become available. Identify that instrument from the following
A. OM counter B. pH counting chamber
C. Flow cytometer D. Nephelometer
68. "Vanillin" a popular flavoring agent for ice cream, is obtained from
A. leaf B. latex
C. bark D. fruit
69. Protein contamination of a DNA preparation is checked by calculating ratio ofabsorbance at wavelengths
A. 280/260 run B. 260/280 run
C. 230/280 run D. 230/260 run
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70. Blocking the active site of an enzyme is a kind of
A. competitive inhibition B. allosteric inhibition
C. non-competitive inhibition D. feed back inhibition
71. Restriction enzyme recognition site refers to
A. specific base sequence of a DNA, which is recognized by a restriction enzyme for its action
B. specific sites in a cell, where restriction enzymes are translated and find their sitc ofaction on DNA
C. specific site on a restriction enzyme, which recognizes a specific DNA sequence for its action
D. specific sites on rough endoplasmic reticulum, where riobosomes
will bind for cotranslational translocation of a protein targeted to endoplasmic reticulum.
72. Match the plant growth regulators from left panel to their effects listed in right panel and mark the correct answer
Plant growth regulator Effects
1. Auxins
I. breaking dormancy ofseeds
M. Cytokinins
II. promotes senescence offlowers
N. Gibberellic acid
III. inhibits the outgrowth of axillary buds
O. Ethylene
IV. prevention of senescence
A. L-III; O-II
B. L-III; 0-1
C. M-III; 0-1
D. M-III; O-II
73. During which phase of the growth of microorganisms the number of dividing cells equal the number ofresting cells
A. Log B. Lag
C. Stationary
D. Death
74. Isotopes have
A. same number of protons but different number of neutrons
B. same number of neutrons but different number ofprotons
C. same number of neutrons but different number ofelectrons
D. same number of protons and neutrons
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75. Fluorescein diacetate is used to test pollen viability based on the activity ofwhich one ofthe following enzymes?
A. Catalase B. Amylase
C. Esterase O. Callose
76. Kinase reactions involve the
A. addition or removal ofa ketone group
B. addition or removal of a phosphate group
C. addition or removal of an amino acid to a polypeptide chain
O. involve the transfer ofhydrogen atoms
77. Bacteria belonging to the genera Myxococcus, Mycobacterium and Streptomyces have one thing in common with reference to the G+C content
A. LowG C content among bacteria
B. High G C content among bacteria
C. 50%G C content
D. G C content
78. Two-dimensional gel electrophoresis is a powerful and widely used method for the analysis of complex protein mixtures extracted from biological samples. This technique can be used to separate proteins according to which ofthe following properties.
First Dimension Second Dimension
A. SDS-PAGE Density
B. Density Charge
C. SDS-PAGE Isoelectrlc focusing
D. Isoelectrlc focusing SOS-PAGE
79. Meselson and Stahl used an isotope to demonstrate semi-conservative nature ofDNA replication. Which isotope did they use?
A. 14C
C. 32p

80. Evolution of CO2 is more than intake ofoxygen when
A. Sucrose is respired B. Glucose is respired
C. Organic acids are respired D. Fats are respired
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81. The first reaction in the pentose phosphate shunt is
A. the oxidation of6-phosphogluconic acid
B. the oxidation ofribose-5-phosphate
C. the oxidation offructose-6-phosphate
D. the oxidation ofglucose-6-phosphate
82. A plant disease in which the pathogen is seen as a cottony growth on the surface ofthe host is called
A. rust B. downy mildew
C. powdery mildew D. smut
83. A lot of starch is deposited in a banana fruit as it matures. Which of the following explains how the starch gets there?
A. Starch solution passes through cells such as companion cell to fruit
B. Starch grains pass through cells from xylem to fruit
C. A sugar solution passes through cells such as companion cells to the fruit where it is changed to starch
D. Starch solution passes through cells from phloem to fruit
84. The drug heroin is prepared by reacting natural product morphine with
A. acetone
B. acetic acid
C. acetic anhydride
D. acetamide
85. Speciation, the process ofspecies formation from an ancestral population, is hastened when
A. the ancestral population remains genetically uniform
B. the ancestral population is genetically diverse
C. when gene flow between two descendent populations is blocked
D. when the two descendent populations continue to interbreed
86. In Drosophila (fruit flies), eye colour is sex-linked and red eye colour is dominant to white eye colour. Which of the following are NOT possible in a cross between a red.:.eyed male and a heterozygous female?
B. White-eyed males A. Red-eyed males D. White-eyed females C. Red-eyed females
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87. Cyanogen bromide is a pseudohalogen compound with a formula CNBr is used to cut protein molecules at the C-terminus of
A. Arginine B. Lysine
C. Glutamic acid D. Methionine
88. Virus accumulations in a host can be studied by tagging its genes by fusing with a reporter gene called Green Fluorescent Protein and can be visualized by using this instrument
A. Common Microscope
B. Ultra Centrifuge
C. Scanning Electron Microscope
D. Confocal Laser Scanning Microscope
89. Which of the following plants has developed a way to circumvent the problems ofphotorespiration?
A. Wheat B. Barley
C. Sugarbeet D. Sugarcane
90. Sulfonamides or sulfa drugs, are structurally related sulfanilamide which
is an analog of one of the following compounds involved in folic acid biosynthesis in bacteria
A. Quinolone B. PABA
C. D. Pentamidine
91. Which ofthe following occurs in the sporangia of mosses?
A. Spores germinate into protonemata
B. Sperm cells are produced in rows just beneath the surface of the antheridiophore
C. Sporocytes undergo meiosis to produce spores
D. The zygote develops into a multicellular embryo
92. The end product of glycolysis or EMP (Embden-Meyerhof-Parnas) pathway and the endproduct ofanaerobic catabolism ofcarbohydrate is
A. Glucose-I-phosphate B. Fructose-I-phosphate
C. Acetyl Co-A D. Pyruvic acid
93. The nucleic acid molecule which acts as a 'dual adaptor' is
A. DNA B. mRNA
C. rRNA D. tRNA
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94. The tissue that makes up most ofthe wood ofa tree is
A. vascular cambium B. secondary xylem
C. primary xylem D. secondary phloem
95. The age ofeyeas plant can be detennined by its
A. growth rings B. size ofits crown
C. amountofcorolloidroots D. leafbasesystem
96. Organisms whose requirement for salt exceeds that ofother organisms
A. Alkalophilic B. Xerophilic
C. Chlorophilic D. Halophilic
97. Growth rings can be seen in plants that grow in
A. Temperate and cold regions
B. Tropical and sub-tropical regions
C. Hot and dry regions
D. Rocky snow clad regions
98. Aspirin and its relatives have been used widely for treatment of fever, pain and inflammation. Chemically aspirin is
A. Paracetamol B. Acetylsalicylic acid
C. Acetaminophen D. Ibuprofen
99. Pollen grains with both a distinct furrow and pore are termed as
A. Colporate B. Porate
C. Colpate D. Synporate
100. Shikonin, the first plant secondary metabolite produced on industrial scale from plant cell cultures is pbtained from
A. Morinda citrifolia B. Hyoscyamus niger
C. Linum jlavum D. Lithospermum erythrorhizon
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