Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharm. SEMESTER V • EXAMINATION WINTER • 2016
Subject Code: 250002 Date: 22-11-2016
Subject Name: Pharmaceutical Microbiology I
Time: 10:30 am 01:30 pm Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Discuss the scope of microbiology in Pharmaceutical industry.
06

Enlist the various microscopic techniques. Describe in brief about SEM.
05

Write a note on Rickttsia.
05
Q.2

Differentiate gram positive and gram negative microorganisms.
06

Classify the various media for bacterial growth. Explain selective
Media.
05

Define 'sterilization'. Explain moist heat sterilization with its mechanism of action.
05
Q.3

Describe various methods used for classification of bacteria. Explain morphological classification of bacteria.
06

Write a note on evaluation of disinfection.
05

Classify the staining techniques. Describe Ziehl-Neelsen staining method
05
Q.4

Classify type of bacteria on the basis of
Nutritional requirement ii) Temperature requirement
06

Enumerate methods for evaluation of disinfectants. Describe Rideal-Walker test
05

Describe biological indicators for monitoring sterilization processes.
05
Q.5

Enumerate the factors affecting on disinfection. Discuss any two.
06

Enlist methods for sterility testing. Discuss direct inoculation method.
05

Describe growth curve of bacteria with labeled diagram.
05
Q. 6

Classify the various methods of bacterial counts. Discuss the plate count technique.
06

Discuss the principle underlying microbiological assay of vitamins.
05

Draw a labeled diagram of bacterial cell. Explain flagella.
05
Q.7

Write significance of sterility testing in pharmaceuticals. Describe methods for sterility testing for sterile gauze.
06

Write a note on validation of filtration sterilization.
05

Write taxonomy of Rickettsia, Actinomycetes.
05