Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
BPharm SEMESTER V EXAMINATION WINTER 2017
Subject code: 250002 Date: 03-11-2017
Subject Name: Pharmaceutical Microbiology I
Time: 10:30 am to 01:30 pm Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

What is Microbiology? Briefly describe the scope of microbiology.
06

Draw typical bacterial growth curve and explain various stages of bacterial growth.
05

Classify bacteria on the basis of:
1. Oxygen requirement
2. Temperature requirement
05
Q.2

Classify methods of bacterial counting. Discuss methods for determining viable count.
06

How the electron microscopy differs from light microscopy? Write in brief on TEM.
05

Enlist the methods for isolation of microorganism. Discuss the streak plate technique.
05
Q.3

Enumerate various factors affecting disinfectant action. Explain any two.
06

Describe in brief the Rideal walker test for the evaluation of disinfectant. Give its advantages and disadvantages.
05

Classify the disinfectants. Write the mode of action of any two.
05
Q.4

Explain the principle of sterility testing. Which media are used for sterility testing?
06

Discuss the ethylene oxide gas sterilization.
05

Write the significance of sterility testing of pharmaceuticals. Discuss the method for sterility testing of soluble powders.
05
Q.5

Classify various methods of sterilization. Describe principle and application of dry heat.
06

Write a short note on Autoclave.
05

Explain the Z and F values with their significance in sterilization.
05
Q.6

Discuss in brief the microbial assay of vitamins.
06

Enlist various methods used for microbial assay. Discuss any one.
05

How the validation of bacterial filters is carried out?
05
Q.7

Classify the staining techniques. Explain the principle of differential staining.
06

Write a brief note on spirochetes.
05

Discuss various techniques used for isolation and cultivation of virus.
05