DISTANCE EDUCATION
M.Sc. (Botany) DEGREE EXAMINATION, MAY 2016.
PLANT TISSUE CULTURE
Time Three hours Maximum 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
1. Give an account on sterilization techniques.
Discuss the stages and uses of micro propagation.
List out the secondary plant products.
2. Discuss about somoclonal variations and mention
the applications.
Give an account on establishment and maintenance
of callus.
List out the significance of Haploid culture.
3. Write an essay on plant growth hormones and their
role in regeneration.
Discuss about the steps involved in synthetic seeds
production.
Write about Embryo rescue.
4. Discuss the procedure for anther culture.
Give an account on meristeum culture.
Write about liquid culture.
Sub. Code
14
DE-3852
2
Sp 1
5. Give an account on Nutrient medic composition.
Discuss about the control of organo genesis and
embryogenesis.
Write about Root culture.
6. Discuss the stages of micro propagation.
Write about somatic embryo genesis.
Write brief notes on totipotency.
7. Outline the commercial importance and
applications of micropropgation.
Give an account on flower bud culture and ovule
culture.
Write notes on meristem culture.
8. Write an essay on protoplast fusion technology.
Enumerate techniques used for in vitro production
of secondary metabolities.
Write notes on mechanical isolation of plant
protoplasts.