DISTANCE EDUCATION
M.Sc. (Botany) DEGREE EXAMINATION, DECEMBER 2017.
(Specialization Plant Bio-Technology)
PLANT TISSUE CULTURE
(2008 Onwards)
Time Three hours Maximum 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
1. Enumerate the steps involved in the establishment
and maintenance of callus and suspension culture.(8)
Discuss the principle and method involved in the
production of Artifical seeds.
Write notes on Meristerm culture.
2. Give a brief account on significance mechanism and
applications of somaclonal variations.
Explain the importance of protoplast fusion and
applications.
Mention about the production of secondary plant
products.
3. Discuss about the auther culture.
Give an account on shoot tip culture.
Write about embryo rescue and applications.
Sub. Code
14
DE-2972
2
Sp 1
4. Discuss about the control of organogenesis and
embryogenesis.
Give an account on protoplast isolation and fusion
methods.
Explain about the flower bud culture.
5. Discuss the various aspects of micropropagation.
Give an account on somatic embryogenesis.
Write about liquid culture.
6. Write about the pollen culture techniques.
How will virus free plantlets through
micropropagation?
Give a brief account on ovule culture.
7. Write notes on root culture and its applications.
Enumerate the various components of solid nutrient
media suitable for plant tissue culture.
Discuss about role of auxins in regeneration of plant
cells.
8. Write an essay on sterilization techniques employed
in plant tissue culture.
What is totipotency? How can we exploit totipotency
in plant tissue culture?