Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
M.PHARM SEMESTER EXAMINATION -WINTER 2018
Subject Code: MPL104T Date: 07/01/2019
Subject Name: Cellular and Molecular Pharmacology
Time :10:30 AM TO 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Explain types of necrosis. Discuss various mechanisms of necrosis.
06

Write principle and applications of PCR.
05

Discuss in detail various mechanisms for termination of GPCR activity.
05
Q.2

Enumerate various intracellular proteins. Explain their functions in detail.
06

Discuss IP3/DAG intracellular signaling pathway.
05

Write applications of recombinant DNA technology.
05
Q.3

Classify voltage sensitive calcium channel. Explain their structure, location and functions.
06

Explain in detail various steps of gene expression.
05

Discuss polymorphism affecting metabolism with suitable examples.
05
Q.4

Explain in detail principle and procedure of SDS-PAGE.
06

Discuss MAPK intracellular signaling pathway.
05

Write applications of gene therapy with special reference to recent advances.
05
Q.5

Discuss intracellular signaling pathway of cytokine receptor.
06

Write principle, procedure and applications of agarose gel electrophoresis.
05

Explain physiological and pathological role of nitric oxide as secondary messenger.
05
Q. 6

Describe techniques for gene transfer. Write a note on viral vectors.
06

Discuss genetic variation in drug transporters with examples.
05

Write short note on autophagy.
05
Q.7

Define cell viability assay. Give principle, procedure and application of MTT assay.
06

Explain cell cycle in detail.
05

Discuss role of apoptosis in various diseases.
05