Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
M.PHARM SEMESTER EXAMINATION -WINTER 2018
Subject Code: 910001 Date: 01/01/2019
Subject Name: Modern Analytical Techniques
Time: 10:30 AM TO 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Define chemical shift. Describe factors affecting the chemical shift.
06

Why C13 NMR spectra are more difficult to record than H-NMR? Describe Spin-Spin decoupling in detail.
05

Discuss principle Instrumentation of NMR.
05
Q.2

Enlist the ionization techniques used in Mass spectroscopy. Discuss CIMS technique.
06

Give fragmentation patterns of following compounds.
Benzyl alcohol Acetone Ethyl secondary butyl ether
05

Describe Mc-Lafferty rearrangement with suitable examples.
05
Q.3

Write principle of X-ray spectroscopy. Explain Bragg's equation for diffraction of X-rays by crystals.
06

Write principle and Instrumentation of ORD.
05

What is plasma? Discuss about Inductively coupled Plasma Emission Spectroscopy.
05
Q.4

Enlist various Thermal Methods. Write a detailed note on TGA with applications.
06

Give differences between ELISA EMIT.
05

Explain principle and application of ZE and IEF
05
Q.5

Which detectors are used in Gas chromatography? Explain working of any one detector.
06

Write notes on (any one)
Super fluid chromatography
ii) Affinity chromatography
05

Write down Instrumentation and applications of HPLC.
05
Q. 6

Enlist the factors affecting the efficiency of chromatographic separation.
Discuss longitudinal diffusion.
06

Give the different between GS-MS LC-MS.
05

Explain Principle and Applications of UV-Visible Spectroscopy.
05
Q.7

Explain terms
Capacity Factor ii) Resolution iii) Shielding effect
06

What is reflection spectroscopy?
Describe ATR with its applications
05

Discuss the principle of atomic absorption spectroscopy and its role in pharmacy.
05