Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
M. Pharm. SEMESTER- I • EXAMINATION SUMMER 2017
Subject Code: 910001 Date: 27/04/2017
Subject Name: Modern Analytical Techniques
Time: 10:30 AM TO 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Explain theory and applications of derivative spectroscopy.
06

Give applications of atomic absorption spectroscopy in Pharmacy.
05

Explain principle and working of mass spectroscopy.
05
Q.2

What is reflection spectroscopy? Describe attenuated total reflection spectroscopy with its applications.
06

Describe with diagram, principle and working of Michelson interferometer.
05

What do you mean by X-ray diffraction? Explain Bragg's law and application of X-ray diffraction.
05
Q.3

Give principle of NMR spectroscopy. How NMR spectroscopy helps in the structure elucidation of compounds?
06

Explain the similarities and differences between proton and C13 NMR.
05

Describe Factors affecting the Chemical Shift.
05
Q.4

Enlist the ionization techniques used in mass spectroscopy. Discuss MALDI technique.
06

Describe Mc-Lafferty rearrangement with suitable examples.
05

Write in brief about optical rotatory dispersion and circular dichroism.
05
Q.5

Explain the term capacity factor, tailing factor and resolution.
06

Explain in detail hyphenation technique LC-MS.
05

What is plasma? Describe inductive coupled plasma emission spectroscopy.
05
Q. 6

Explain the terms of Van Demeter equation. Describe eddy and longitudinal diffusion in detail.
06

Explain the principle and instrumentation of Supercritical Fluid chromatography.
05

What is HPLC? Explain the principle of HPLC.
05
Q.7

Enumerate different methods of Thermal analysis. Explain DSC in detail.
06

What are reference standards? Discuss sources, storage and records for reference standards.
05

What is Radio Immuno Assay? Discuss principle and method used in ELISA technique.
05