Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM SEMESTER EXAMINATION -WINTER 2018
Subject Code: 2240003 Date: 12/12/2018
Subject Name: Pharmaceutical Chemistry V (Biochemistry II)
Time: 02:30 PM TO 05:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Discuss the pyrimidine biosynthesis with its regulation.
06

Explain any two mechanism of Oxidative phosphorylation.
05

Explain Electrophoresis as a tool of Biochemistry.
05
Q.2

Describe in detail transamination and deamination reactions for amino acids metabolism.
06

Write a Short Note on denaturation of Protein.
05

Give detail account on Operon Concept.
05
Q.3

Write about the reactions involved in the heme biosynthesis.
06

Explain Southern Blotting technique as a tool of analysis in Biochemistry.
05

Define Protein and classify proteins based on chemical nature.
05
Q.4

What is the importance of bile pigments in human body? How it is formed and what are the implications of its metabolic disorders.
06

Outline the step involve in the urea cycle and state its significance.
05

Write application of genetic engineering.
05
Q.5

Write a note on biosynthesis of RNA.
06

Discuss polymerase chain reaction in detal.
05

Notes on biological significance of ATP and cyclic AMP.
05
Q. 6

Write a note on gene expression in eukaryotes.
06

Write a short note on Genetic code.
05

Explain briefly about biosynthesis of nucleic acid.
05
Q.7

What is replication? Why DNA replication is called semi-conservative. Explain the role of DNA polymerase, DNA ligase and DNA helicase in replication of prokaryotes.
06

Explain about excision-repair of DNA.
05

Discuss in detail isolation and purification of nucleic acid.
05