Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM SEMESTER EXAMINATION -WINTER 2018
Subject Code:2250002 Date: 20/11/2018
Subject Name: Pharmaceutical Microbiology Biotechnology I
Time:10:30 AM TO 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Classify Bacteria based on Shape, O2 requirement and Flagellar arrangement with suitable examples.
06

Explain various Dry mount techniques for identification of Microbes.
05

Differentiate Scanning and Transmission Electron Microscopy.
05
Q.2

Give the reasons for the following:
alcohol is more effective than 90% alcohol ii) Meaning of "oligo" in oligodynamic action of metals iii) Instead of Straight line, Sigmoidal curve is observed in dynamics of disinfection.
06

Write short notes on Redeal Walker test.
05

Give a brief account on Bacterial Growth curve.
05
Q.3

Describe the construction and working of Horizontal Autoclave with a neat labeled diagram.
06

Explain the terms: Synchronous growth, Tyndallization, Pasteruization, Cold Sterilization and Sintering
05

Write short notes on Sterilization using Depth Filters.
05
Q.4

Enumerate Methods of purifying the cultures.
06

Explain the cycle of Dormant body formation from a vegetative cell with a neat diagram.
05

Write short notes on Virus Cultivation.
05
Q.5

Describe the steps involved in DNA Replication.
06

Write short notes on Triplet codon and mention the features of Genetic Code.
05

Give a brief account on Scope of Biotechnology.
05
Q. 6

Explain immobilization techniques in detail.
06

Write short notes on Criticism of Phenol Coefficient Tests.
05

Give a brief account on Taxonomy of Actinomycetes.
05
Q.7

Classify different types of media with suitable examples.
06

Mention the Biological Indicators used for various sterilization processes and criteria for selection as Biological Indicator.
05

Write short notes on factors affecting Disinfectants.
05