Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharm SEMESTER-V EXAMINATION SUMMER 2017
Subject Code: 2250002 Date: 29/05/2017
Subject Name: Pharmaceutical Microbiology Biotechnology I
Time: 10:30 AM to 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Discuss the Scope of Microbiology
06

Enumerate methods for evaluation of disinfectants. Describe Rideal-Walker test.
05

Explain: value, value, value, Q10- value, Inactivation factor
05
Q.2

Classify the microorganisms. Differentiate the bacteria and viruses.
06

Discuss electron microscopy with its applications
05

Describe biological indicators for monitoring sterilization processes
05
Q.3

Classify the staining techniques. Discuss gram staining method
06

Discuss in Detail about genetic code and its properties
05

Write a brief note on Spirochetes.
05
Q.4

Classify the disinfectants. Discuss the mechanism of action of any two.
06

Differentiate between: capsule and spore
Selective and differential media
05

Discuss factors affecting disinfection.
05
Q.5

Write a note on an autoclave.
06

Draw a labeled diagram of bacterial cell. Explain flagella.
05

Write a note on gaseous sterilization
05
Q. 6

Define total counts. Enlist methods for total count and discuss any one.
06

Explain various techniques of immobilization
05

Discuss the nutritional requirement of microbes.
05
Q.7

Explain salient features of enzyme immobilization.
06

Describe the structure of DNA and the process of DNA replication
05

Write short note on scope of biotechnology
05