Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM.-SEMESTER-VIII- EXAMINATION -SUMMER-2017
Subject Code: 280004 Date: 04/05/2017
Subject Name: Pharmaceutical Analysis IV
Time: 10:30 AM to 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Explain the principle and applications of Raman Spectroscopy.
06

Discuss the applications of X-ray diffraction technique.
05

Write a note on nephelometry.
05
Q.2

Discuss the theories of chromatography.
06

Write applications of GC and GC-MS.
05

Define: (i)Retention time (ii)Tailing factor (iii)Capacity factor (iv)Selectivity factor (v)Resolution
05
Q.3

Differentiate between: (i)Analytical and guard column (ii)Normal and reverse phase chromatography (iii)Isocratic and gradient elution.
06

Explain principle behind ion-exchange chromatography. Discuss the factors affecting retention in this technique.
05

Explain the principle of separation in size exclusion and affinity chromatography.
05
Q.4

With a suitable diagram, describe the components of a gas chromatograph.
06

Describe flame ionization detector in gas chromatography.
05

Give an account of various stationary and mobile phases used in GC.
05
Q.5

Compare and contrast advantages and limitations of HPLC with HPTLC.
06

Discuss the various applications of TLC and HPTLC.
05

Describe the instrumentation in HPTLC.
05
Q. 6

Define Intellectual property Limit of detection Accuracy Precision Rf value Calibration
06

Discuss the requirements for an invention to be patentable.
05

Write a note on supercritical fluid chromatography.
05
Q.7

Name the different radiochemical methods of analysis. Explain in detail any one of them.
06

Explain the principle and advantages of RIA.
05

Describe the steps for filing a patent application in India.
05