FACULTY OF PHARMACY
M. Pharmacy (Pharmaceutical Analysis) II-Semester (Suppl.) Examination,
February 2019
Subject: Modern Bio Analytical Techniques
Time: 3 Hours Max. Marks: 75
Note: Answer any five questions. All questions carry equal marks.
1 Write about the following sample preparation techniques. 6
Solid phase extraction
Liquid Liquid extraction
Explain the Bioanalytical method validation as per USFDA guidelines. 9
2 Discuss about Biopharmaceutical factors affecting drug bioavailability. 10
Write the Biopharmaceutics classification system defined by FDA. 5
3 What is enzyme inhibition? Discuss about drug interactions due to enzyme
inhibition with examples. 7
Discuss about drug-protein binding interactions with examples. 8
4 Write about principles, instrumentation and applications of flow cytometry. 9
Write about cryopreservation and storage of cells. 6
5 Explain different study designs in bioequivalence studies. 10
Differentiate absolute and relative bioavailability with illustrative examples
and equations. 5
6 Discuss the importance and applications of Toxicokinetic studies. 8
Write about basic equipments used in cell culture lab. 7
7 Discuss about different approaches for identification of metabolites. 10
Write short note on clinical significance of bioequivalence studies. 5
8 Describe the compendia methods of dissolution testing. 7
Write about in-vivo and in- vitro methods for checking cellular permeability
of new drug products. 8