FACULTY OF PHARMACY
M. Pharmacy (Pharmaceutical Analysis) II-Semester (Suppl.) Examination,
February 2019
Subject: Advance Instrumental Analysis
Time: 3 Hours Max. Marks: 75
Note: Answer any five questions. All questions carry equal marks.
1 Write the principle involved in HPLC and explain the following.
Peak shapes Plate number Plate height
Explain various pumps used in HPLC.
2 Explain the principle and stationary phases of the following: (2x7½)
Ion Exchange chromatography Affinity chromatography
3 Write in detail about Instrumentation, columns and detectors used in Gas
chromatography.
4 Explain the instrumentation and applications of super critical fluid
chromatography.
Explain characteristics and pharmaceutical analysis of capillary
electrophoresis.
5 Explain the following ionization techniques
chemical ionization FAB ESI
Explain fragmentation pattern of
Alcohols Aldehydes aliphatic acids
6 Explain the following:
Spin-spin coupling
Coupling constant
Nuclear magnetic double resonance
7 Write about the principles instrumentation and applications of (2x7½)
TLC
Size exclusion chromatography
8 Explain in detail about chiral stationary phases
Explain principle and applications of HPTLC.