(Pages 1378
P.T.O.
MAR IVANIOS COLLEGE (AUTONOMOUS)
THIRUVANANTHAPURAM
Reg. No. :.………………… Name :.…………………
Second Semester Career Related B.Sc. Degree Examination, June 2016
First Degree Programme under CBCSS Foundation Course II: (for Botany and Biotechnology)
AUBB221: Biophysics and Instrumentation
Time 3 Hours Max. Marks 80
SECTION A
Answer ALL questions in a word or one or two sentences.
1. Enthalpy and entropy.
2. MALDI TOF.
3. Redox potential.
4. Molar extinction coefficient.
5. Partition Principle in chromatography.
6. Absorption spectra.
7. Beer Lamberts law.
8. Condenser in microscopes.
9. Principle of Electrophoresis.
10. Radio isotopes.
(10 × 1 10 Marks)
SECTION B
Answer any EIGHT questions, not exceeding a paragraph.
11. Thermodynamic equilibrium.
12. Phase contrast microscopy.
13. Two dimensional electrophoresis.
14. Ultra centrifugation.
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15. Mechanism of Vision.
16. Chemi osmotic hypothesis.
17. Adsorption Chromatography.
18. Fluorimetry.
19. Mass spectrometry.
20. Phosphorescence and its importance.
21. Light harvesting pigments in chloroplast.
22. Endothermic and exothermic reactions in biological system.
× 2 16 Marks)
SECTION C
Short essay type Answer any SIX questions.
23. Give an outline of mechanism involved in hearing.
24. Phase contrast microscopy.
25. Differential gradient Centrifugation.
26. UV VIS Spectrophotometry.
27. Autoradiography.
28. Radio isotope tracer techniques.
29. Give an outline of energy change in biochemical reactions.
30. Photophosphorylation.
31. X Ray crystallography.
× 4 24 Marks)
SECTION D
Long essay type Answer any TWO questions.
32. Give the mechanisms involved in NMR Spectrometry.
33. Explain the Principle and process of HPLC in detail.
34. Explain SDS PAGE.
35. Instrumentation and working Principle of Electron Microscope.
× 15 30 Marks)