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P.T.O.
MAR IVANIOS COLLEGE (AUTONOMOUS)
THIRUVANANTHAPURAM
Reg. No. :.………………… Name :.…………………
Second Semester Career Related Degree Examination, June 2016
First Degree Programme under CBCSS Foundation Course II: (for Botany and Biotechnology)
AUBB221: Biophysics and Instrumentation
Time 3 Hours Max. Marks 80
SECTION A
Answer ALL questions in a word or one or two sentences.
1. Give an example of iodine radioisotope used as therapeutic agent.
2. Define isoelectric point.
3. Explain Beer Lambert law.
4. Which is the primary electron donor in Photosystem I
5. Explain van der Waals force.
6. What is the function of condenser lens in a microscope
7. What is the function of Iris in human eye
8. What is photophosphorylation
9. What is an endothermic reaction
10. Define pH.
(10 x 1 10 Marks)
SECTION B
Answer any EIGHT questions, not exceeding a paragraph.
11. What is Gibbs free energy
12. Explain autoradiography.
13. What is phosphorescence
14. Draw the absorption spectra of chlorophyll.
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15. What is meant by resolving power of microscope
16. Explain density gradient centrifugation.
17. Explain chemi osmotic hypothesis.
18. What are the applications of SDS electrophoresis
19. What is astigmatism
20. Explain the first law of thermodynamics.
21. What is the biological importance of hydrophobic interactions
22. Explain ionizing radiations.
x 2 16 Marks)
SECTION C
Short essay type Answer any SIX questions.
23. Explain isoelectric focusing.
24. Explain X ray crystallography.
25. What are the applications of radioisotopes in biological research
26. Explain rocket immunoelectrophoresis.
27. What is the use of phase contrast and fluorescence microscope in biological research
28. Write notes on the light harvesting pigments in photosynthesis.
29. Explain atomic absorption spectroscopy.
30. How does a hearing aid work
31. How ATP synthesis occur in mitochondria
x 4 24 Marks)
SECTION D
Long essay type Answer any TWO questions.
32. Explain the principle, working and applications of TEM.
33. Write notes on NMR spectroscopy and its application in biological science.
34. Explain ultracentrifugation.
35. Explain Acrylamide gel electrophoresis.
x 15 30 Marks)