N A L A N D A O P E N U N I V E R S I T Y
M.Sc. Zoology, Part-II
PAPER-X
(Biotechnology)
Annual Examination, 2016
Time: 3 Hours. Full Marks: 80
Answer five questions in all, selecting at least Two Questions from each Group.
All Questions carry equal marks.
GROUP
1. Describe the working principle of Spectrophotometer giving its two applications.
2. Describe Southern blotting technique. Give an account of major steps involved in isolation of a
particular gene from genome.
3. Discuss principles and basis of scanning electron microscopy.
4. Give an account of steps involved in DNA amplification method for RNA essay and significance
of RNA assay.
5. Write notes on any Two of the following:—
Radioisotopes
HIV virus
Beta Decary
Radiation detecting devices.
GROUP
6. Write an essay on RIA and its application.
7. What is fixation? Give an account of nature of fixatives and properties of chemical fixing
reagents.
8. Describe comet assay technique for detection of apopototic cells.
9. Discuss the labeling antibodies.
10. Write notes on any Two of the following:—
Staining
Biosensor
SDS-PAGE
TLC