N A L A N D A O P E N U N I V E R S I T Y
M.Sc. Zoology, Part-II
PAPER-X
(Biotechnology)
Annual Examination, 2018
Time: 3 Hours. Full Marks: 80
Answer five questions in all, selecting at least Two Questions from each Group.
All Questions carry equal marks.
GROUP
1. Explain the procedure and advantages of fluorescence method by DNA assay.
2. Explain the Southern blotting in detail.
3. Discuss the advantage of vacuum blotting over capillary blotting.
4. "Raman spectroscopy is good Scientific Research tool" Justify your answer in detail.
5. Write notes on any Two of the following:—
Radiation detecting devices.
Limitations of fluorescence microscope
Stage scanning and Beam scanning
Modern confocal microscope
GROUP
6. What do you understand by transmission electron microscopy Discuss its structure and function.
7. What is apoptosis Describe briefly comet Assay technique for the detection of apoptic cells.
8. Give the steps name involved in the preparation of Permanent Slide.
9. Write the description of basic feature of HPLC and its applications.
10. Write notes on any Two of the following:—
Write the types of absorbent used during TLC
Nuclear and plasma stains
Immunosensor
Write the basic principle of RIA