Biotechnology(Semester (CBCS) Examination, 2017
BIOINFORMATICS
ANIMAL BIOTECHNOLOGY AND STEM CELL TECHNOLOGY
wateriI
Day Date: Wednesday, 19-04-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
N.B. 1)Section I compulsory
Answer any four questions from section-II
SECTION-I
Q.1 Multiple Choice Questions 07
The cell line used for the production of Polio vaccine

Primate Kidney cell line CHO cell line
Dog kidney cell line Mouse Fibroblast cell line
2 The Virus commonly used to infect cell cultures for the
production of interferons is
Corona virus Sendai virus
Polio virus Small pox virus
3 A heterologous protein for its expression in the milk of a
transgenic animal should be under the control of the gene
coding for
Preproinsulin Lac Z globin lactoglobin
4 Aminopterin is used during the production of hybridoma cells
because it
Blocks the Salvage pathway.
Prevents the growth of B cells.
Prevents the growth of myeloma cells.
Blocks the synthesis of Ig by B cells.
5 First cloned animal was
Dolly sheep Dog Mule Cat
6 Transgenic goats have been used to produce the protein
used to dissolve blood clots for
Amyloid precursor
a1-antitrypsin
Casein
A variety of human tissue type Plasminogen activator.
7 The Father of Animal cell culture was
Ross Harrison Whatson
Page 2 of 2
Johnson Chris Harris
SLR-RD 136
Define the following terms: 07
Contact inhibition
Continuous cell line
Feeder layers
Asepsis
Cryopreservation
Organ culture
Stem cell
Section-II
Q.2 Briefly explains the design of an animal cell culture bioreactor and
types.
14
Q.3 Define Organotypic culture and write in detail haw they are made. 14
Q4 Describe in detail the types of grafts used for organ transplantation
and its applications.
14
Q5 Answer any two from the following 14
Methods of Preservation of cell cultures.
Serum free media.
CO2 incubator
Q.6 Answer any two of the following 14
Primary culure.
Mode of cell and tissue delivery.
Applications of animals cell culture in day life.