M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Biotechnology
ANIMAL BIOTECHNOLOGY AND STEM CELL TECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: Section I is compulsory.
Answer any four questions from section II.
Draw neat labeled diagram wherever necessary.
SECTION I
Q.1 Rewrite the sentences by choosing correct answer from given
alternatives:
07
Medullary plate of chick Embryo was maintained by Roux in
Warm Saline Plasma clots
Agar clot Serum
E S Cell lines in culture were first obtained by
IVF Mouse Blastocysts
Chick Embryo Germ Cells
Liquid phase temperature for very long period of storage of cells is

-70 oC -120 oC
-196 oC -110 oC
The quality and quantity of wool can be improved in sheep by the supply of
aminoacids.
Serine Methionine
Lysine Cysteine
Neural stem cells cultured in vitro is called
Neuronal cells Glial cells
Neurospheres EG cells
The first human cell line to be grown continuously in the laboratory is

Hela cell line MRC 5
3T3 cell line HLM cell line
is a favorite animal for harvesting human organ for organ
transplant.
Chicken Pig
Sheep Goat
Define the terms:- 07
Passage number
Organotypic culture
Grid Technique
Gene Knock Out
Cryotubes
Cell Lines
Hayflick effect
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SLR-VE-88
SECTION II
Q.2 Write in details about the different type of cell culture media, its growth
supplement and its advantages.
14
Q.3 Write in details about Hybridoma technique and its importance. 14
Q.4 Explain in detail culture of cells for production of various biological. 14
Q.5 Answer any two of the following: 14
What is organ culture? Write about the techniques used in organ culture.
Discuss the importance characteristics of stem cells.
Write a note on cryopreservation methods.
Q.6 Answer any two of the following: 14
Explain in detail about immunoisolation techniques.
Write a note on scale up in monolayer culture with diagram.
Discuss the different types of Tissue culture and its advantages.