M.Sc. (Semester IV) (CBCS) Examination Oct/Nov-2017
Biotechnology
ANIMAL BIOTECHNOLOGY AND STEM CELL TECHNOLOGY
Day Date: Friday, 17-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: All questions of Section I is compulsory.
Answer any Four questions from section II.
Section I
Q.1 Multiple choice Questions:- 07
Animal cell cultures are used widely for the production of
Insulin Somatostatin
MABS Thyroxine
The first vaccine developed from animal cell culture was for
Hepatitis B Influenza
Small Pox Polio
Recombinant proteins are
Proteins synthesized in animals
Proteins synthesized by transgene in host cells by r DNA technique
Proteins synthesized in cells that are produced by protoplast fusion.
Proteins synthesized in mutated cells line
Interferons are
Antibacterial proteins Anti viral proteins
Bacteriostatic proteins All of these
The production of complete animals from somatic cells of an animal is
called
a)a Gene cloning Animal cloning
Cell cloning All of these
In Bioreactors are used for aeration.
Spargers Impellers
Baffles Both B C
Which of the following has been produced commercially from
mammalian cultures
Insulin Renin
Plasminogen activator Antibacterial antibody
Define the following terms:- 07
Suspension culture
Scaffolds
Scaling up
Cell line
Cryopreservation
Stem cells
Xenograft
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Section II
Q.2 Give brief account on the Bioreactor design of Animal cell culture with the help
of a suitable diagram.
14
Q.3 Write in details about the behavior of cells in culture. 14
Q.4 Discuss the concept of Transgenic animal technology and write about the
methods used for preparing transgenes.
14
Q.5 Answer any two from the following 14
Hematopoietic Stem cells.
Regeneration of bone and cartilage.
Animal cell culture medium
Q.6 Answer any two of the following 14
Common cell culture contaminants.
Significance of Knock out animals.
Organotypic Culture.