M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Biotechnology
ANIMAL BIOTECHNOLOGY AND STEM CELL TECHNOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Section I compulsory. Answer any Four questions from section II.
Section I
Q.1

Multiple choice Questions:-
07
First cloned animal was
Dolly sheep
Dog
Mule
Cat
Transgenic goats have been used to produce the protein used to dissolve blood clots for
Amyloid precursor protein
1-antitrypsin
Caesin
A variety of human tissue type Plasminogen activator
The Father of Animal cell culture was
Ross Harrison
Whatson
Johnson
Chris Harris
Animal cell cultures are used widely for the production of
Insulin
Somatostatin
MABS
Thyroxine
The first vaccine developed from animal cell culture was for

Hepatitis B
Influenza
Small Pox
Polio
Recombinant proteins are
Proteins synthesized in animals
Proteins synthesized by transgene in hose cells by r DNA technique
Proteins synthesized in cells that are produced by protoplast fusion
Proteins synthesized in mutated cell line
Interferons are
Antibacterial proteins
Anti Viral proteins
Bacteriostatic proteins
All of these

Define the following terms:-
07
Suspension culture
Scaffolds
Scaling up
Cell line
Cryopreservation
Stem cells
Xenograft
Page 2 of 2
SLR-UE-102
Section II
Q.2
Briefly explains the design of an animal cell culture bioreactor and its types.
14
Q.3
Define Organotypic culture and write in detail how they are made.
14
Q.4
Describe in detail the types of grafts used for organ transplantation and its applications.
14
Q.5
Answer any two from the following
14
Methods of preservation of cell cultures.
Serum free media.
CO2 incubator.
Q.6
Answer any two of the following
14
Primary culture.
Mode of cell and tissue delivery.
Applications of animal cell culture in day to day life.