M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Microbiology
BIOENERGETICS AND MOLECULAR ENZYMOLOGY
Day Date: Tuesday, 21-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: Part Q. 1 is compulsory
Attempt any four questions from part II.
Figures to the right indicate full marks.
PART-I
Q.1 Rewrite the following sentences by selecting correct answers from given
alternatives:-
14
The atoms of the pyrmidine ring are derived from
Glutamine
Carbamoyl phosphate and Aspartate
Glucose
Glutamic acid
is substrate specific enzyme.
Hexokinase Thiokinase
Lactase Decarboxylase
are end products of aerobic respiration.
Sugar &O2 Amino acid and NAD+
NADH2 FADH2 CO2, water and energy
The plot between reciprocal of v and is known as plot.
Lineweaver-Burk Hanes
Eadie Hofstec
is a key intermediate compound linking glycolysis to Kreb's
cycle.
Pyruvic acid Acetyl CoA
Citric acid Malate
The enzymes involved in feed back inhibition are called
enzymes.
Allosteric Co
Apo Holo
is precursor for all sterols.
Terpene Lipid
Glycolipid Cholesterol
All enzymes are proteins with exception of
RNAase RNA polymerase
RNA ligase Ribozyme
are Sudanophillic granules.
Glycogen Starch
PHB Volutin
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SLR-MQ-719
10) Enzymes of the class are involved in synthesis of new
band.
Transferases Ligases
Lyases Hydrolases
11) molecules of ATP are produced by the aerobic oxidation of
one molecule of glucose.
38 2
12 8
12) Enzymes are
Thermophile Thermostable
Thermolabile Thermopstatic
13) The kinetic models to explain enzyme catalysed reactions was proposed
in 1913 by
Khune Kholand Hanes and Edie
Hanes and Menten Michaelis Menten
14) involved in conversion of Nucleosides to nucleotides.
Nucloside oxidase Nucloside kinase
Nucleoside Transferase Nucloside reductase
PART -II
Q.2 Describe in detail TCA cycle 14
Q.3 Discuss in detail derivation of Michaelis menten equation and its significance
in Enzyme Kinetics.
14
Q.4 Describe in detail biosynthesis of purines and Pyrmidines. 14
Q.5 Attempt any two of the following:- 14
EMP pathway.
Microbial degradation of aliphatic hydrocarbons.
Enzyme as biocatalyst.
Q.6 Attempt any two of the following 14
Bacterial Fermentations.
Degradation and regulation of reserve materials.
Classification of Enzymes.