M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Microbiology
BIOENERGETICS AND MOLECULAR ENZYMOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
Partial or incomplete oxidation of substrate occurs during
Respiration Fermentation
Anabolism None of these
Microbial Renin is used in
Lipolysis of milk Stormy fermentation
Curdling of milk Putrefaction of milk
When an inhibitor binds selectively to complex and not to the free enzyme
it brings about inhibition.
Irreversible Competitive
None Competitive Uncompetitive
An example of ligase is
Succinate thiokinase Alanine racemase
Fumarase aldolase
An inducer is absent in type of enzyme.
Allosteric Constitutive
Isoenzyme Co-operative
In Non competitive inhibition, inhibitor
Increases Km Decreases Km
Does not affect Km None of these
The rate of TCA cycle is controlled by the
Aconitase Fumarase
Isocitrate dehydrogenase Malate dehydrogenase
enzyme reaction is not irreversible in glycolysis.
3-phosphoglycerate kinase Hexokinase
Glucokinase Pyruvate kinase
is the rate determining step of Michaels Menten Kinetics.
Complex dissociation step to produce product
Product formation step
Complex formation step
Enzyme formation step
10) acts directly on an enzyme to lower it's catalytic rate.
Inhibitor Regulator
Repression Moderator
11) Breaking of Terminal phosphate of ATP releases about Kcal energy.
6.3 6.1
7.1 7.3
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SLR-VP-439
12) Catalytic efficiency of two distinct enzymes can be compared based on
Km Product
Substrate Enzyme
13) The enzyme tyrosinase is activated by
Iron Copper
Zink Potassium
14) amino acid undergoes oxidative deamination at a highest rate.
Glutamine Glutamate
Aspartate Alanine
Q.2 Answer the following any four:-
What is Pasteur effect?
Conversion of ribonucleotides into deoxyribonucleotides.
What is substrate level phosphorylation?
What is significance of PHB granules?
Write on significance of L.B. Plot.
08
Write short notes (Any two
Glycerol metabolism
Mixed acid fermentation
Mechanism of action of Ribonuclease.
06
Q.3 Answer the following:- (Any Two)
Describe biosynthesis of purines.
Describe ED pathway.
Discuss the role of active site in enzyme catalysis.
08
Answer the following. (Any One)
Discuss the factors affecting rate of enzyme mediated reactions.
Describe microbial degradation of aliphatic hydrocarbons.
06
Q.4 Answer the following:- (Any Two)
Describe in detail acetone butanol fermentation.
Degradation of aromatic compounds.
Discuss enzymatic synthesis degradation of glycogen.
10
Answer the following. (Any One)
Explain different properties of enzymes.
Glyoxylate pathway.
04
Q.5 Answer the following. (Any Two) 14
Discuss in detail classification of enzymes with examples.
Derive Michaelis Menten equation.
Describe in detail EMP pathway.