M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Microbiology
HEALTH CARE AND DIAGNOSTIC MICROBIOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Part- Questions No.1 Compulsory. Attempt any 4 questions from Part II. Figures to the right indicate full marks. Answers to Part-I and Part-II are to be written in same answer booklet only.
PART I
Q.1
Rewrite the sentences after choosing correct answer from the given alternatives:-
14
causes ADP ribosylation of elongation factor 2 leads to inhibition of proteins synthesis in target cells.
Diphtheria toxin
Botulinum toxin
Escherichia coli endotoxin
Erythrogenic toxin
Which of the following antibiotic affect protein synthesis
Actinomycin D
Chloramphenicol
Sulphonamides
Vancomycin
Which of the following is NOT true for exotoxins?
The exotoxins can work by binding and entering the host cell
They can cause toxaemia
They rarely have enzymetic activity
They can be converted to toxoids
Chloramphenicol antibiotic inhibits synthesis.
Cell wall
Protein
Membrane
DNA
Scientist who discovered penicillin was
Alexander Flemming
Walksman
Benet
Burnet
Which of the following enzyme is used in Enzyme linked immunoabsorbant assay?
Protease
Lipase
Amylase
Peroxidase
Endotoxins have relatively potency.
Low
High
Same
No
Endotoxins are part of the outer membrane of the cell wall of
Gram positive bacteria
Gram negative bacteria
Actinomycetes
Fungi
s
Adenylate cyclise toxin is produced by
Vibrio cholarae
Bordutella pertussis
Chlostrium tetani
Corynebacterium diphtheriae
Page 2 of 2
SLR-UQ-551
10) Which of the following bacterium produce neurotoxin which is A B toxin cause flaccid paralysis?
C. diphtheriae
V. choleriae
C. tetani
S. aureus
11) Exotoxins are typically
Proteins
Carbohydrates
Lipids
Sugars
12) Which of the following is NOT a semi synthetic chemotherapeutic agent?
Ampicillin
Penicillin
Carbanicillin
Trimethoprim
13) Gentamicin is an antibiotic produced by
Micromonospora
Penicillium
Streptoverticillium
Streptromyces
14) acts as folic acid intermediate antagonists.
s
Trimethoprim
Sulphonamides
Both a and b
None of these
PART II
Attempt any four questions from the part II
14
Q.2
Write in brief on "Mechanism of action of antibiotics inhibiting protein synthesis".
Q.3
Write in short on "Use of antigen antibody reaction used in medical diagnosis".
14
Q.4
Write in detail on "Immunohistochemistry and agglutination reaction".
14
Q.5
Write in short on any Two of the following:
14
Various microbial enzymes causing antibiotic resistance in bacteria.
Florescence In Situ Hybridization (FISH)
Adhesion and invasion
Q.6
Write short notes on any Four of the following:
14
Antibiotic sensitivity testing by disc and MIC method
Differentiate between Endotoxins and Exotoxins
Polymerase chain reaction and its use in diagnosis of disease.
Action of Cycloheximide
What is Gradient plate technique? Write its application.
What is FDA?