M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Microbiology
HEALTH CARE AND DIAGNOSTIC MICROBIOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: Part-I, question 1 is compulsory.
Attempt any four questions from Part II
Figures to the right indicate full marks.
Answers to the Part I and Part II are to be written in same answer
book.
PART I
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
acts as folic acid intermediate antagonists.
Trimethoprim Sulphonamides
Both a and b None of these
Gentamicin is an antibiotic produced by
Streptomyces Penicillium
Streptoverticillium Micromonospora
Which of the following is NOT a semisynthetic chemotherapeutic agent?
Chloramphenicol Penicillin
Carbanicillin Ampicillin
Exotoxins are typically
Carbohydrates Proteins
Lipids Polysaccharides
Which of the following bacterium produces neurotoxin causing flaccid
paralysis?
C. tetani V. choleriae
C. diphtheriae S. aureus
Streptomycin binds subunit of bacterial ribosome.
60 s 50 s
40 s 30 s
shows synergistic action with Trimethoprim.
Penicillin Streptomycin
Sulphonamides Tetracyclin
Amphotericin B acts on
Bacteria Actinomycetes
Fungi Viruses
is the mechanism of antibiotic resistance.
Decreased cell wall permeability to antibiotics
Enzymatic deactivation of antibiotics
Altered target sites of antibiotic
All of these
Page 2 of 2
SLR-VP-443
10) Adenylate cyclase activity is observed with toxin
Cholera Diphtheria
Tetanus Endotoxins
11) Streptomycin antibiotic inhibits synthesis
Cell wall Protein
Membrane DNA
12) Which of the following is NOT true for exotoxins?
They can be converted to toxoids
They rarely have enzymetic activity
They can cause toxaemia
The exotoxins can work by binding and entering the host cell
13) Which of the following antibiotic affect protein synthesis?
Actinomycin D Vancomycin
Chloramphenicol Sulphonamides
14) causes ADP ribosylation of elongation factor 2 leads to inhibition
of protein synthesis in target cells.
Diphtheria toxin Botulinum toxin
Escherichia coli endotoxin Erythrogenic toxin
PART II
Attempt any four questions from part II.
Q.2 Write in detail on 'Mechanism of action of antibiotics inhibiting protein synthesis'. 14
Q.3 Write in detail on 'Agglutination and complement fixation test and its application' 14
Q.4 Write in detail on 'Enzyme linked immunosorbant assay'. 14
Q.5 Write in short note (Any Two) 14
Adhesion and invasion
Florescence In Situ Hybridization (FISH)
Various microbial enzymes causing antibiotic resistance in bacteria.
Q.6 Write short notes (Any Two) 14
Polymerase chain reaction.
Differentiate between Endotoxins and Exotoxins.
Antibiotic sensitivity testing by disc method.