M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Microbiology
PRINCIPLES OF BIOINSTRUMENTATION AND TECHNIQUES
Time: 2½ Hours
Max. Marks: 70
Instructions: Part Question 1 is compulsory. Answer any 4 questions from Part II. Figures to the right indicates full marks Answer to the Part I and Part II are to be written in same answer booklet only
Part I
Q.1
Rewrite the sentence by choosing correct alternative from the following:
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microscopy sample interact with the wavelength of light to produce high contrast image without need of dye and no damage to the sample
Conventional bright filed light microscopy
Electron Microscopy
Phase contrast microscopy
Fluorescence microscopy
In ion exchange chromatography, if protein is stable at values below the isoelectric point, exchanger should be used.
Anion
Cation
Mixed
All of these
The GC trace obtained after an experiment is called
Chromatograph
Chromatogram
Chromatophore
Graph
In 2D gel electrophoresis the final gel separates the proteins on the basis of
pI and MW
pH and Molarity
Charge and MW
Charge and pI
For creating pH gradient in IEF are used.
Ampholytes
SDS
Polyacrylamides
Detergents
In NMR when nuclei are placed in magnetic field, all the random
Stop
Changed in reverse directions
Align with the magnetic field
Rotate 90°C away from the induced field
Electrons of Scanning Electron Microscope are reflected through
Glass funnel
Specimen
Metal-coated surfaces
Vacuum chamber
Magnification of light microscope is
1500X
2000X
1000X
2500X
Page 2 of 2
SLR-UQ-550
Affinity chromatography deals with the
Specific binding of a protein constituent for another molecule
Protein protein interaction
Protein carbohydrate interaction
Protein lipid interaction
10) are considered to be the lowest form of Electromagnetic radiation?
IR radiation
Micro waves
UV radiation
Radio waves
11) is a source used in spectroscopy.
LASER
Tube light
Sodium vapour lamp
Tungsten lamp
12) is the formula for pH calculation.
log10[H+].

log2[H+].

13) Why is the computer necessary in Fourier Transform Spectrometer?
To display the detector output
To process the detector output
To determine the amplitude
To determine the frequency
14) sensor is used in ESR spectrometer?
Hall-effect sensor
Load cell
Strain gauge
Bourdon gauge
Part II
Q.2
What is the principle of flow cytometry? Explain in detail working of flowcytometry and comment on its applications.
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Q.3
State Beer and Lambert's law. Explain the working of U.V. Visible spectrophotometer and give its applications.
14
Q.4
Which factor affects electrophoretic mobility of molecule? Explain principle and working of SDS-PAGE.
14
Q.5
Write short answer (Any Two)
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Rate zonal and Isopycnic density gradient
Fluorescence Microscopy
Principle and application of ion exchange chromatography
Q.6
Write short answer (Any Two)
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Explain western blotting technique and give its significance.
Comment on method of freeze etching and freeze fracturing.
Comment on HPLC.