M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Microbiology
PRINCIPLES OF BIOINSTRUMENTATION AND TECHNIQUES
Time: 2½ Hours Max. Marks: 70
Instructions: Part Q.1 is compulsory.
Attempt any four questions from part-II.
Figures to the right indicate full marks.
Answer to the Part I and Part II are to be written in same answer booklet
only.
Part I
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
is used to visualize live cells.
SEM TEM
Phase contrast Cytometer
is best suited to get the surface view of an object.
SEM TEM
Phase contrast Compound
The GC trace obtained after an experiment is called
Chromatograph Chromatogram
Chromatophore Graph
Thin layer chromatography is
Partition chromatography Electric mobility of ionic species
Adsorption chromatography High pressure chromatography
In reversed phase HPLC there is a
Non polar solvent polar solvent
Polar solvent/ Non polar solvent
Non polar solvent non polar solvent
Polar solvent polar solvent
gas is unsuitable for use as carrier gas in GC.
Nitrogen Helium
Oxygen All of the above
Electrons of Scanning Electron Microscope are reflected through
Glass funnel Specimen
Metal-coated surface Vacuum chamber
IEF is widely applied for
Drug testing Protein purification
Protein identification Protein digestion
Acrylamide concentration of the stacking gel is always than that
of the resolving gel.
Greater Lesser
Same May be greater or lesser
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SLR-VP-442
10) The compound added to disrupt the disulfide bonds present in the proteins is

TEMED APS
Beta mercaptoethanol SDS
11) is a source used in spectroscopy?
LASER Tube light
Sodium vapour lamp Tungsten lamp
12) is the formula for pH calculation?
log10[H+].
log2[H+].
13) is the x-axis of a mass spectrum?
mass mass/energy
mass/charge charge
14) Technique is suitable for identifying mRNA.
Western blotting Southern blotting
Northern blotting Eastern blotting
Part II
Q.2 What is the principle of Affinity chromatography? Explain in detail working of
Affinity chromatography and give its applications.
14
Q.3 What is circular dichroism? Explain the instrumentation of CD and give its
applications.
14
Q.4 Which factor affects electrophoretic mobility of molecule? Explain principle and
working of agarose gel electrophoresis.
14
Q.5 Write short answer to (any 14
Give working and applications of ultra centrifuge machine.
What is MALDI and ESI? What is its role in MS?
Explain principle and application of TEM.
Q.6 Write short notes on (any 14
Explain Northern blotting technique and give its significance.
Comment on method of freeze etching and freeze fracturing.
Comment on Phase contrast Microscopy.