M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Microbiology
HEALTH CARE AND DIAGNOSTIC MICROBIOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: Part Question 1 is compulsory.
Attempt any four questions from part II.
Figures to the right indicate full marks.
Answers to part I and part II are to be written in same answer booklet only.
PART I
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
is a glycopeptides antibiotic used in the prophylaxis and
treatment of infections caused by gram positive bacteria.
Azasurin Vancomycin
Chloramphenicol Chloroquin
Antigenic substance present on surface of Escherichia coli is
K antigen M protein
H antigen O polysaccharide
toxin is plasmid encoded.
Cholera Diphtheria
Tetanus Endotoxin
toxin is an inhibitor of protein synthesis.
Cholera Diphtheria
Tetanus Endotoxin
Erythromycin binds sub unit of bacterial ribosomal r RNA.
60 S 23 S
50 S 30 S
Trimethoprim and sulphonamides inhibit
Protein synthesis DNA RNA function
Cell wall synthesis Folic acid biosynthesis
toxins are responsible for fever production in human being.
Tetanus toxin
Endotoxins of gram negative bacteria
Cholera toxin
Diphtheria toxin
Most antibiotics are produced by
Viruses Plants
Fungi Actinomycetes
Beta lactams ring is present is antibiotic.
Vancomycin Chloramphenicol
Semi synthetic Penicillins Tetracycline
10) is a penicillinase resistant antibiotic.
Amoxycillin Amoxicillin
Rifamycin Methicillin
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SLR-VP-445
11) is a cell wall synthesis inhibitor antibiotic.
Vancomycin Polymixin
Chloramphenicol Rifamycin
12) is an invasions produced by Clostridium spp which dissolves
collagen framework of muscles.
Coagulase Collaginase
Leucocidin Streptolysin
13) A disease „whooping is caused by the organism
Vibrio cholerae Bacillus anthracis
Bordetella pertussis Streptococcus pyogenes
14) antibiotic chemotherapeutic agent is very difficult to produce by
synthetic method.
Erythromycin Chloramphenicol
Vancomycin Neomycin
PART II
Attempt any four questions from Part II
Q.2 Write in brief on "Virulence factors of bacteria". 14
Q.3 Write in short on "Polymerase chain reaction and steps involved in PCR
experiment".
14
Q.4 Write in detail on "Mechanisms of action of diphtheria and cholera toxin." 14
Q.5 Write short answer to (any 14
Enzyme linked Immunosorbent Assay (ELISA)
Determination of Minimum inhibitory concentration
Antibiotic sensitivity testing
Q.6 Write short notes on (any 14
What is Complement fixation test? Write its use in diagnosis.
How antibiotic inhibit protein synthesis to kill bacteria?
Role of microbiology laboratory in quality control in industry.
Purification and characterization of bioactive molecules.
Penicillin and Vitamin B12 assay.
What is Carcinogenicity and teratogenicity testing of a drug?