M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Microbiology
BIOPROCESS TECHNOLOGY AND FERMENTATION TECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
is a downstream process.
Screening Product recovery
Sterilization of media Inoculum preparation
The cell density in is controlled by increasing and decreasing
flow of culture medium.
Chemostat Turbidostat
Continuous culture Synchronous culture
Transformation of progesterone to 11∝ hydroxyprogesterone is carried out
by
S. griseus C. lunata
P. niger R. nigricans
Streptomycin fermentation by streptomyces griseus requires temperature
between range.
0 5 10 15
25 30 35 40
In whisky the soured mash may be heated to
55 63
65 None of these
Nucleosides are fermented by using mutant strain of
B. Subtilis Brevi bacterium ammoniagenes
S. Cerevisiae Asp. Oryzae
Recovery of alcohol is carried out by
Chromatography Distillation
Precipitation Adsorption
Allergy testing is carried out on
Mice Rabbit
Cat Horse
In fermentation process agitation of medium is done by
Impellers Antifoam agents
Baffles Spargers
10) Protein content of mushrooms is about of dry weight.
25 30% 1
50 60% 80 90%
Page 2 of 2
SLR-VP-437
11) In the industrial production of streptomycin, the secondary metabolite or by
product is
Vit. B12 Vit. C
Vit. B6 Ethanol
12) The term stands for
Word Investment Policy Organization
Word Intellectual Property Organization
Wildlife Investigation Policy organization
World Institute for prevention of organized crime
13) is used for industrial production of amylase.
B. amylolyticus Vibrio Cholerae
B. Polymyxa E. Coli
14) Batch fermentation is also called
Closed system Fed batch system
Open system Submerged System
Q.2 Answer the following any four:-
1. Enlist types of fermenters.
2. What is Reynolds number?
3. What is principle of continuous fermentation?
4. Write on secondary metabolites with examples.
5. Brief on strain improvement.
08
Write notes on any two
1. Scale up of fermentation
2. Solvent extraction
3. Carcinogenicity testing
06
Q.3 Answer the following (Any
1. Industrial production of L. Iysine.
2. Describe steroid transformation.
3. Discuss mushroom production with respect to
Harvesting, preservation nutritive value
08
Answer the following (Any
1. Industrial production of Whisky.
2. Describe in detail biosafety levels of infections agents
06
Q.4 Answer the following (Any
1. Describe assay testing of products.
2. Designing of fermentation media.
3. Sterilization of fermenter and other mechanical system.
10
Answer the following (Any
1. Production of Dextran.
2. Guidelines for safety in microbiology laboratory.
04
Q.5 Answer the following (Any 14
Describe in detail production of Vit. B12
Write an essay on Bioethics.
Describe in detail automation in fermentation Industry.