B.Sc. III (Sem. VI) (Entrepreneurship) (CGPA) Examination, 2018
GENETIC ENGINEERING AND TECHNIQUES IN PLANT TISSUE CULTURE
Day and Date Wednesday, 4-4-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
N.B. All questions are compulsory and carry equal marks.
Draw a neat labeled diagram wherever necessary.
Figure to the right indicates full marks.
1. Rewrite the following sentences by choosing correct alternatives from given
below 14
is an excised piece of leaf or stem tissue used in micropropagation.
Microshoot Medium Explants Scion
ii) Plant tissue culture technique is a redefined method of
Hybridization Vegetative propagation
Selection None of these
iii) Restriction endonucleases are used in genetic engineering, because it cut
the DNA and produce
Two fold symmetry SS DNA
DS DNA None of these
iv) is used surface cleaning agent of seed or plant material.
NaCl HgCl2 KCl HCl
Somatic hybridization is achieved through
Conjugation Grafting
Protoplast fusion Isolated ovary culture
vi) is called as the father of plant tissue culture.
P. R. White C. D. Larue
J. P. Nitsch Gotlib Hyberlandt
vii) Totipotency is the genetic potential of a plant cell to produce
Only leaf Only fruit Only shoot Entire plant
viii) The first hormone at industrial level produced by using bacteria is
Insulin thyroxin
testosterone none of these
ix) The most widely used chemical for protoplast fusion, as fusogens is
Mannitol Sorbitol
Polyethylene Glycol Lysosomes
First step in genetic engineering is
Isolation of RNA Isolation of protein
Isolation of genetic material Purification of protein
xi) The first transgenic crop is
Maize Tobacco Jowar Wheat
xii) A medium which is chemically composed of specifically defined components
is called as
Synthetic media Seminatural media
Natural media None of these
xiii) The technique of obtaining identical copies of the desired gene is called as

Gene therapy Gene sequence
Gene cloning rDNA technology
xiv) vector is mainly used in crop improvement.
Mitochondrion Cosmid
Plasmids Agrobacterium
2. Answer any seven of the following 14
Define Bacteriophage.
ii) Define Genetic Engineering.
iii) Transgenic plants.
iv) Applications of PCR.
What is callus
vi) Restriction Endonucleases.
vii) Ligases.
viii) cDNA Library.
ix) Flavor savor tomato.
3. Answer any two of the following 10
Describe in brief Nutritional requirements of the explants.
ii) Discuss in detail PCR.
iii) Describe in detail History and Ethical issues of genetic engineering.
Give a detailed account on PGR. 4
4. Answer any two of the following 14
Describe in detail Western blotting.
ii) Explain in detail Enzymes involved in genetic engineering.
iii) Explain in detail Suspension Culture.
5. Answer any two of the following 14
Write in detail Micro propagation and its applications.
ii) Discuss in detail PAGE.
iii) Describe in detail applications of Plant Tissue Culture with example.