Exam Details
| Subject | (genetic engineering and techniques in plant tissue culture) | |
| Paper | ||
| Exam / Course | b.sc. – i (entrepreneurship) | |
| Department | ||
| Organization | solapur university | |
| Position | ||
| Exam Date | October, 2018 | |
| City, State | maharashtra, solapur |
Question Paper
B.Sc. III (Semester VI) Entrepreneurship (cgpa) Examination, 2018
ind. microbiology and ind. biotechnology
(Genetic Engineering and Techniques in Plant Tissue Culture)
Day and Date Friday, 2-11-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Choose the correct alternative and rewrite the sentences again. 14
The plant part used for tissue culture is known as
Callus Explant
Tissue All of above
ii) The unorganized dividing mass of tissue produced during tissue culture
process is known as
Explant Callus Tissue None of these
iii) The nutrient which are most essential and required in large amount for
tissue culture process.
Micronutrient Macronutrient
Salts Mineral nutrients
iv) is the genetic vector.
Pili Cosmid
Flagella All of above
Plasmid chiefly occurs in
Viruses Bacteria
Chromosomes Chloroplast
vi) hormone is used in tissue culture process.
Auxin and Cytokinin Gibberellins and Cytokinin
Auxin and Gibberellins Ethylene and Abscisic acid
Seat
No.
P.T.O.
Set P
vii) Genetic engineering is
Plastic surgery Addition and removal of genes
Study of extra-nuclear genes All of above
viii) Restriction endonuclease is
enzyme nucleic acid
fatty acid polysaccharide
ix) Small circular self replication and extra chromosomal and double standard
DNA molecule present in bacterial cell is
Chromosome Plasmid
Bacteriophage Virus
For production of haploid plant is used as explant.
Anther Leaf Stem Root
xi) is used for sterilization of glass wares.
Autoclave Laminar air flow
Hot air oven Disinfectant
xii) For tissue culture process is mainly used media.
M.S. media White media
Chu media None of these
xiii) The computer based study and designing of genome is called
lipidomics proteiomics
polymics genomics
xiv) is the "Molecular scissors" used to genetic engineering.
DNA polymerase DNA ligase
Restriction endonuclease Helicase
SLR-SK 56 *SLRSK56*
Set P
*SLRSK56* SLR-SK 56
2. Define and explain in short any seven of the following. 14
Agarose
ii) Insects resistance.
iii) PTC.
iv) Nucleases.
Anther culture.
vi) Cellular totipotancy.
vii) Ovule culture.
viii) Explants.
ix) Callus.
3. Answer any two of the following. 10
Write note on Organ culture with suitable diagram.
ii) Describe in detail Transgenic plants.
iii) Write note on Suspension culture with Synchronization.
Write note on Cosmid with suitable example. 4
4. Answer any two of the following. 14
Explain in detail Western blotting with diagram.
ii) Describe in detail factors affecting the micropropogation.
iii) Explain in detail bacteriophage and phagemid vector with diagram.
5. Answer any two of the following. 14
Describe in detail ovule and ovary culture with diagram.
ii) Explain in detail construction of chimeric DNA with preparation and labeling.
iii) Describe in detail plant growth hormones.
ind. microbiology and ind. biotechnology
(Genetic Engineering and Techniques in Plant Tissue Culture)
Day and Date Friday, 2-11-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Choose the correct alternative and rewrite the sentences again. 14
The plant part used for tissue culture is known as
Callus Explant
Tissue All of above
ii) The unorganized dividing mass of tissue produced during tissue culture
process is known as
Explant Callus Tissue None of these
iii) The nutrient which are most essential and required in large amount for
tissue culture process.
Micronutrient Macronutrient
Salts Mineral nutrients
iv) is the genetic vector.
Pili Cosmid
Flagella All of above
Plasmid chiefly occurs in
Viruses Bacteria
Chromosomes Chloroplast
vi) hormone is used in tissue culture process.
Auxin and Cytokinin Gibberellins and Cytokinin
Auxin and Gibberellins Ethylene and Abscisic acid
Seat
No.
P.T.O.
Set P
vii) Genetic engineering is
Plastic surgery Addition and removal of genes
Study of extra-nuclear genes All of above
viii) Restriction endonuclease is
enzyme nucleic acid
fatty acid polysaccharide
ix) Small circular self replication and extra chromosomal and double standard
DNA molecule present in bacterial cell is
Chromosome Plasmid
Bacteriophage Virus
For production of haploid plant is used as explant.
Anther Leaf Stem Root
xi) is used for sterilization of glass wares.
Autoclave Laminar air flow
Hot air oven Disinfectant
xii) For tissue culture process is mainly used media.
M.S. media White media
Chu media None of these
xiii) The computer based study and designing of genome is called
lipidomics proteiomics
polymics genomics
xiv) is the "Molecular scissors" used to genetic engineering.
DNA polymerase DNA ligase
Restriction endonuclease Helicase
SLR-SK 56 *SLRSK56*
Set P
*SLRSK56* SLR-SK 56
2. Define and explain in short any seven of the following. 14
Agarose
ii) Insects resistance.
iii) PTC.
iv) Nucleases.
Anther culture.
vi) Cellular totipotancy.
vii) Ovule culture.
viii) Explants.
ix) Callus.
3. Answer any two of the following. 10
Write note on Organ culture with suitable diagram.
ii) Describe in detail Transgenic plants.
iii) Write note on Suspension culture with Synchronization.
Write note on Cosmid with suitable example. 4
4. Answer any two of the following. 14
Explain in detail Western blotting with diagram.
ii) Describe in detail factors affecting the micropropogation.
iii) Explain in detail bacteriophage and phagemid vector with diagram.
5. Answer any two of the following. 14
Describe in detail ovule and ovary culture with diagram.
ii) Explain in detail construction of chimeric DNA with preparation and labeling.
iii) Describe in detail plant growth hormones.
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- basics of biomolecules (paper – i)
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