B.Sc. II (Entrepreneurship) (Semester IV) (New CBCS) Examination, 2018
Microbial Biotechnology
Molecular Biology (Paper VII)
Day and Date Tuesday, 8-5-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
1. Rewrite the correct alternative from the following questions 14
In A DNA base pairs are present in per turn of the helix.
10 11 12 13
The base thymine is always paired with
Adenine Guanine Cytosine Thymine
Uvr ABC endonuclease is present in system.
Mismatch repair Nucleotide excision repair
Base excision repair SOS repair
amino acid has maximum genetic codone.
Leucine Tryptophan Valine Alanine
subunit of E.coli RNA polymerase is responsible for gene
selection.
Alpha Beta Omega Sigma
is not found in an E.coli replication fork.
DnaA
Primase
PCNA
Single-stranded DNA binding protein
Full expression of the lac operon requires
lactose and cAMP allolactose and cAMP
cAMP lactose
DNA polymerase III is thought to add nucleotides
to the end of the RNA primer
to the end of the RNA primer
in the place of the primer RNA after it is removed
on single stranded templates without need for an RNA primer
Enzyme that induces double strand breaks in DNA and rejoins them is
called as
Restriction endonuclease DNA gyrase
DNA ligase DNA polymerase
10) Ultraviolet radiation causes DNA damage by formation of
cytidine dimer thymidine dimer
guanine dimer adenine dimer
11) Archea is considered as a separate group from bacteria and eukaryotes
based on
genome sequence 16S rRNA gene sequence
23S rRNA gene sequence EFTu sequence
12) Okazaki segments are small pieces of DNA containing 1000 to 1500
nucleotides and are formed in
lagging strand sense strand
leading strand non sense strand
13) In bacterial promoters, describes the 'Pribnow box'.
The untranslated region The-35 box
The-10 box The termination sequence
14) DNA polymerase is required for the synthesis of
DNA from DNA RNA from RNA
RNA from DNA DNA from RNA
2. Answer any seven of the following 14
DNA Polymerase in prokaryotes.
Topoisomerase.
Draw the Structure of DNA.
The Central Dogma.
Ribosome structure.
RNA polymerase in eukaryotes.
Silent of DNA.
Operators.
Central Dogma.
3. Answer any two of the following 10
Explain Genetic code and its properties.
Describe in detail Nucleotide Excision repair system in prokaryotes.
Write a detail note on Translation in prokaryotes.
Write a detail note on Replication in prokaryotes. 4
4. Answer any two of the following 14
Describe in brief Transcription in Prokaryotic Cell.
Write a detail note on Rolling Circle Model of DNA.
Explain in detail Lac Operon.
5. Answer any two of the following 14
Briefly explain types of DNA.
Describe in brief Enzyme involved in eukaryotic Replication.
Write a essay on Post transcriptional modification.